{"metadata":{"accession":"A0AA96ZY71","id":"A0AA96ZY71_9EURY","source_organism":{"taxId":"3028294","scientificName":"Methanimicrococcus stummii","fullName":"Methanimicrococcus stummii"},"name":"DNA topoisomerase 1","description":["Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone"],"length":772,"sequence":"MYLIITEKDTAAKRIAAILSENKVKKVRYPKLGIETYEFTYNDDHTVVMGLSGHVVGIDFPSEYNNWQKNLARDLIDAEIIPISTNKKIVTALKVIGKKADLVTIATDYDREGELIGVEALTLVQSVNPDIKFNRALYSAITPKDITHAFENLTTVNFDLADAGHAREVIDLVWGAALTRYISLCAGRLGKLFLSVGRVQSPTLALIVEREKEREAFIPVQYWEIETTLETKSNESFIAEYHTNRIFEKEIAERVYSNVVVGNDAKIKSIEAKKRKEKPPIPFNTTEFYVAANAVGISVNNAKRMYQTLYEAGYLSYPRTDSTIYPESLELRSLIEMFVGTEFDTYAKALLKIADKDGLKPTQGKKEDPAHPPIHPVAPAKRTDMKEDEWKIYELVVRRFFATFAGEAEWDTMKVEIDINSEEFKSNGSRLTEEGWRWYYPYNKPEDRVLPHLEEGGLLKITHKELFDKETQPPSRYGQGRLIRIMDDMGIGTKATRADIINKLVSRYYVHGNPLQPTNMAYSVIDTLEQYAPTIVKPDMTKQLEADMDKISEGQISEAIVLKESREMLNNIFDELEKNHEDIGRSLQSGLREDKIVGKCQRCGSDLIIRKSKKGSRFIGCSGYPDCDFSLPVPRTGQVVVTDKMCDDHQMYKIKIITEGKRPWELGCPHCNFIEWQKKVEEDEKNGIVAETSAERAKRIRKEKAAAKAEREAKAANPTELSDIKGLGTASLEKLNDAGVMTLSDLASADSGKLAKETGISLKNIEKWKEVL","proteome":"UP001302662","gene":"topA_1","go_terms":[{"identifier":"GO:0003677","name":"DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003916","name":"DNA topoisomerase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006265","name":"DNA topological change","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0005694","name":"chromosome","category":{"code":"C","name":"cellular_component"}},{"identifier":"GO:0000166","name":"nucleotide binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003917","name":"DNA topoisomerase type I (single strand cut, ATP-independent) activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"28aad0d1fb2b41e8e413ccc00dab079a2ea69ab9","counters":{"domain_architectures":96,"entries":42,"isoforms":0,"proteomes":1,"sets":5,"structures":0,"taxa":1,"dbEntries":{"smart":3,"profile":2,"cdd":2,"cathgene3d":6,"ssf":3,"pfam":4,"hamap":1,"ncbifam":2,"panther":1,"prints":1,"prosite":1,"interpro":16},"proteome":1,"taxonomy":1,"similar_proteins":96}}}