{"metadata":{"accession":"A0A8T2E4M6","id":"A0A8T2E4M6_9BRAS","source_organism":{"taxId":"1240361","scientificName":"Arabidopsis thaliana x Arabidopsis arenosa","fullName":"Arabidopsis thaliana x Arabidopsis arenosa"},"name":"Iron-sulfur cluster assembly protein","description":["Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU (ISU1, ISU2 or ISU3). In a second step, the cluster is released from ISCU, transferred to a glutaredoxin, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH"],"length":167,"sequence":"MMLKQAAKKALGLTSRQSTPWSVGILRTYHENVIDHYDNPRNVGSFDKNDPNVGTGLVGAPACGDVMKLQIKVDEKTGQIVDARFKTFGCGSAIASSSVATEWVKGKAMEDVLTIKNTEIAKHLSLPPVKLHCSMLAEDAIKAAVKDYKEKRVKTNGAAAAGETTQA","proteome":"UP000694240","gene":"ISN45_At04g023330","go_terms":[{"identifier":"GO:0005506","name":"iron ion binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0051536","name":"iron-sulfur cluster binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0016226","name":"iron-sulfur cluster assembly","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"1a14465eba7d2532ef70113b6acb6327d7aa9427","counters":{"domain_architectures":26938,"entries":8,"isoforms":0,"proteomes":1,"sets":2,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cathgene3d":1,"cdd":1,"panther":1,"pfam":1,"ncbifam":1,"interpro":2},"proteome":1,"taxonomy":1,"similar_proteins":26938}}}