{"metadata":{"accession":"A0A3B3TM62","id":"A0A3B3TM62_9TELE","source_organism":{"taxId":"48699","scientificName":"Poecilia latipinna","fullName":"Poecilia latipinna (sailfin molly)"},"name":"Abasic site processing protein HMCES","description":["Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The HMCES DNA-protein cross-link is then either reversed or degraded. HMCES is able to catalyze the reversal of its thiazolidine cross-link and cycle between a cross-link and a non-cross-linked state depending on DNA context: mediates self-reversal of the thiazolidine cross-link in double stranded DNA, allowing APEX1 to initiate downstream repair of abasic sites. The HMCES DNA-protein cross-link can also be degraded by the SPRTN metalloprotease following unfolding by the BRIP1/FANCJ helicase. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine"],"length":362,"sequence":"MCGRTACTLAPDEVSRACSYRNRGGQRRQPRWRDGDADKYRPSYNKSLQSMSPVLVSQRHFDKTAPVDECVLASMRWGLIPAWFKESDPSKMQYSTSNCRSENILEKKSYKDPLLKGQRCVIMADGFYEWQRVEKEKQPFFIYFPQKNGPSQERKEDQQQDVTSSTCNTVVSEKGEISHDLAEESEAPAEWTGWRLLTMAGVFDCWTPPGGGEALYTYSVITVNASPNLQSIHDRMPAILDGEDEVRRWLDFGEVKSLDAIKLLQSKDVLTFHPVSSFVNNSRNNSPECLQPVDLSRKKEIKATASSKMMMSWLSSGSPAKRKEPDTNETKGEQDSKAKSRCKPAGGLQLWLQGANKKPRTK","proteome":"UP000261500","gene":null,"go_terms":[{"identifier":"GO:0003697","name":"single-stranded DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006974","name":"DNA damage response","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0106300","name":"protein-DNA covalent cross-linking repair","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"547c439dfd7afa9f6ed8bb0f63dd9ad6a6f27c6d","counters":{"domain_architectures":26373,"entries":6,"isoforms":0,"proteomes":1,"sets":0,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"ssf":1,"pfam":1,"panther":1,"interpro":2},"proteome":1,"taxonomy":1,"similar_proteins":26373}}}