{"metadata":{"accession":"A0A397HK89","id":"ANKE_ASPTH","source_organism":{"taxId":"41047","scientificName":"Aspergillus thermomutatus","fullName":"Aspergillus thermomutatus"},"name":"NRPS-independent siderophore synthetase-like protein ankE","description":["NRPS-independent siderophore synthetase-like protein; part of the ank cluster that mediates the biosynthesis of NK13650 C, a highly modified cyclo-arginine-tyrosine dipeptide (PubMed:36702957). AnkE is responsible of the production of NK13650 B via ligation of citrate to the ankD product (PubMed:36702957). Within the pathway, the cyclodipeptide synthase ankA acts as the scaffold-generating enzyme and is responsible for formation of the cyclo-Arg-Tyr diketopiperazine (cRY) from L-Arg and L-Tyr. The ankA product cRY is desaturated by the cytochrome P450 monooxygenase ankB to yield a dehydro-cyclodipeptide intermediate. The FAD-dependent monooxygenase ankC then installs the m-OH, ankD catalyzes the attachment of L-homoserine, and ankE ligates citrate to the ankD product to yield NK13650 B. The O-methyltransferase ankF is responsible for methylation of the C-17 phenol group of NK13650 B to produce NK13650 D. Amidation of NK13650 D with L-Asp by ankG then leads to the production of NK13650 C, whereas amidation of NK13650 B produces NK13650 A (PubMed:36702957)"],"length":604,"sequence":"MTVPPISERASFEVAKRLLCQILNEGLVSGTLETFESEEPYLCLFRNSCNTEKPEKCIRVRLQPSAQAGIFMRSARIVSLVRPEMLQMPVTLVDGTSERRELRSGALFQFASSLFTEHVEASTLEEIALGLENSEANTEKWLELHSSPGPLDLNSPSVLWERALIWGHPTHPFHRLCYAQEGLAPVEPRDLPEFLTPTLAFVSVLRDEISISGPFEESLQPLLAQLDVPAPETADRVVVPCLRLQLPCVFRHFPSAVLVKTVSGCADTQASMRTLTLRPELNFPYHIKLSIACQITSDVRAIRPCQTLGGQLVRKQLHEFFPPDLWWFKEVASVAGNQTAQGDTQENEDKARHIGCILREDLEARANANNEALIIAGSLAQQPANDPRTHAEIVFGLETVEQKHDWLKEYVAGFLRAVLPSLVQYGIGMEAHGQNALVRVCRDTRKITGFVVRDFEGIKVHVPTLEKLGINLCMTSPGCTKNLEDIWSKIHHSIFQNHLGNLVYALGLDRHDGWTIIRDELFAALKPDTDPRAKELYDFILQDTMPFKCFLRMRMSEHFNDYDGDEQDQPRVKRDERPLPNALLMGSARWEKVLNECAPKKLES","proteome":"UP000215305","gene":"ankE","go_terms":[{"identifier":"GO:0019290","name":"siderophore biosynthetic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":1,"source_database":"reviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"4fd13fc20de0c23c20dba0905d0dcdeeaf05f543","counters":{"domain_architectures":12564,"entries":7,"isoforms":0,"proteomes":1,"sets":1,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":1,"panther":1,"pfam":2,"interpro":3},"proteome":1,"taxonomy":1,"similar_proteins":12564}}}