{"metadata":{"accession":"A0A371NYR6","id":"A0A371NYR6_9ACTN","source_organism":{"taxId":"2292704","scientificName":"Aeromicrobium endophyticum","fullName":"Aeromicrobium endophyticum"},"name":"L-threonine dehydratase","description":["Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA"],"length":420,"sequence":"MSPTPLSPVTAASIDQAAVRVAEVVSRTPLELNARLSRATGAQVWLKREDLQPVRSYKIRGAYNLIVQLDDDTRARGVVCASAGNHAQGVALACRRLGVHGRIFVPGTTPRQKRERIVTLGGSGVEVVVVGDSYEDAFRAAHDDSVATGAALVPAFDDPRTIDGQGTVASEIVDQLGSAPDLVVLPVGGGGLIAGMSTWMDERHPSTRIVGVEPQGAASMTAALAAGAPVAIESVDPFVDGAAVGTVGRHTFPLVRDAGATMVTVDEGHLCTEMLLLYQSDGIIAEPAGALATAALDGLIDVEPGSTVVCVVSGGNNDISRYGEILERSLIHRGLKHYFLVSFPQEPGALRRFLDEILGPDDDITLFDYVKRNNRENGPALIGIELARAEDLAGLLGRLDDSPLGVERVEPGSPMFHYLI","proteome":"UP000265581","gene":"ilvA","go_terms":[{"identifier":"GO:0004794","name":"threonine deaminase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0030170","name":"pyridoxal phosphate binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006520","name":"amino acid metabolic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"ida_accession":"32e92e134fa084251f00c97018c34211af570ede","counters":{"domain_architectures":5053,"entries":18,"isoforms":0,"proteomes":1,"sets":3,"structures":0,"taxa":1,"dbEntries":{"cdd":1,"ssf":1,"cathgene3d":2,"pfam":2,"profile":1,"ncbifam":2,"panther":1,"prosite":1,"interpro":7},"proteome":1,"taxonomy":1,"similar_proteins":5053}}}