{"metadata":{"accession":"A0A2S4LRK4","id":"A0A2S4LRK4_9BURK","source_organism":{"taxId":"1189126","scientificName":"Paraburkholderia eburnea","fullName":"Paraburkholderia eburnea"},"name":"L-threonine dehydratase","description":["Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA"],"length":437,"sequence":"APNLSARLRNTVLLKREDNQPVFSFKLRGAYNKMAHLSAEQLQRGVITASAGNHAQGVALSAARLGVKAVICVPVTTPQVKVDAVRAHGGSMVEVVQAGESYSDAYAHAVRLQEERSLTFVHPFDDPYVIAGQGTVAMEILSKHQAPIHAIFVPIGGGGLAAGVAAYVKAVRPEIRVIGVQTDDSCAMAQSLKAGKRVELSEVGLFSDGTAVKLVGEETFRLCQEYLDDVLTVDTDALCAAIKDVFQDTRSVLESAGALAVAGAKQYAEREGIDDKTLIAITSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEERGSFKRFCELVGTRSVTEFNYRIDDAERAHIFVGVQIRNRGESQQIAEAFVTHGFPSVDLTGDELSKQHIRYMVGGRSPLAHDERLFRFEFPERPGALMRFLSSMAPNWNISLFHYRNQGA","proteome":"UP000237381","gene":"ilvA","go_terms":[{"identifier":"GO:0004794","name":"threonine deaminase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0009097","name":"isoleucine biosynthetic process","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0030170","name":"pyridoxal phosphate binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006520","name":"amino acid metabolic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":true,"in_alphafold":true,"in_bfvd":false,"ida_accession":"dca8c35a0c02c1a2752d0ea9189b163faeded61c","counters":{"domain_architectures":9770,"entries":22,"isoforms":0,"proteomes":1,"sets":4,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":2,"cdd":2,"ssf":2,"pfam":2,"profile":1,"ncbifam":3,"panther":1,"prosite":1,"interpro":8},"proteome":1,"taxonomy":1,"similar_proteins":9770}}}