{"metadata":{"accession":"A0A2C9EMS7","id":"A0A2C9EMS7_PSEPH","source_organism":{"taxId":"1124983","scientificName":"Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CFBP 6595 / CHA0)","fullName":"Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CFBP 6595 / CHA0)"},"name":"L-threonine dehydratase","description":["Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA"],"length":345,"sequence":"MSRPSRSATPQPSADMALLERYVKMILAAPVYDLAIRTPLQAAPALSERLGNQILLKREDLQPTFSFKIRGAYNRLAHLTPEQKLRGVVTASAGNHAQGVALAARELGIKASIVMPASTPELKVLGVRSRGAEAILHGQSFPFALEHALDLAQRTGSTFVSPFDDPHVIAGQGTVAMEILRQQQGELDAIFVPVGGGGLIAGIAAYVKYLRPQIRIVGVESEHSNCLQQALAAGQRVVLPEVGTFADGVAVAQIGEHGFQICRHHVDQVISVSNDQLCAAIKDIYDDTRSITEPSGALAVAGIKEYVAQSGVRGQTLVAIDSGANINFDSLRHVAERAALSNAAL","proteome":"UP000013940","gene":"ilvA1","go_terms":[{"identifier":"GO:0004794","name":"threonine deaminase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:1901705","name":"L-isoleucine biosynthetic process","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0030170","name":"pyridoxal phosphate binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006520","name":"amino acid metabolic process","category":{"code":"P","name":"biological_process"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"ida_accession":"8bc361cd7c1d6142f8798dedc919bc5496ed8194","counters":{"domain_architectures":134083,"entries":12,"isoforms":0,"proteomes":1,"sets":2,"structures":0,"taxa":1,"dbEntries":{"ssf":1,"cdd":1,"cathgene3d":1,"ncbifam":1,"panther":1,"pfam":1,"prosite":1,"interpro":5},"proteome":1,"taxonomy":1,"similar_proteins":134083}}}