{"metadata":{"accession":"A0A0K0HAM4","id":"A0A0K0HAM4_SALBC","source_organism":{"taxId":"218493","scientificName":"Salmonella bongori (strain ATCC 43975 / DSM 13772 / NCTC 12419)","fullName":"Salmonella bongori (strain ATCC 43975 / DSM 13772 / NCTC 12419)"},"name":"DNA topoisomerase 1","description":["Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone"],"length":865,"sequence":"MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWDAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFDKPGELNIDRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEIDANTTTPSGDALPLQVTHQNDKPFRPVSREQTLAAISLLEKARYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPDNPNQYASKENSQEAHEAIRPSDVSVMAESLKDMEADAQKLYQLIWRQFVACQMTSAQYDSTTLTVGAGEFRLKARGRILRFDGWTKVMPALRKSDEDRTLPAVNKGDALTLLELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMEDSLDQVANHQAEWKAVLDNFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALSPKERCKTTINLVPENEVLNVLEGDDAETNALRAKRRCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGIFLAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTVIRFSRKTKQQYVAAEKEGKATGWSAFFVDGKWVEGKK","proteome":null,"gene":"topA","go_terms":[{"identifier":"GO:0003677","name":"DNA binding","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0003916","name":"DNA topoisomerase activity","category":{"code":"F","name":"molecular_function"}},{"identifier":"GO:0006265","name":"DNA topological change","category":{"code":"P","name":"biological_process"}},{"identifier":"GO:0005694","name":"chromosome","category":{"code":"C","name":"cellular_component"}},{"identifier":"GO:0003917","name":"DNA topoisomerase type I (single strand cut, ATP-independent) activity","category":{"code":"F","name":"molecular_function"}}],"protein_evidence":3,"source_database":"unreviewed","is_fragment":false,"in_alphafold":true,"in_bfvd":false,"ida_accession":"84eb3345bda88e5c4f9a133b760e8e1f470f9ad0","counters":{"domain_architectures":4098,"entries":41,"isoforms":0,"proteomes":0,"sets":4,"structures":0,"taxa":1,"dbEntries":{"cathgene3d":6,"smart":3,"profile":2,"cdd":2,"ssf":2,"pfam":5,"panther":1,"hamap":1,"ncbifam":1,"prosite":1,"prints":1,"interpro":16},"proteome":0,"taxonomy":1,"similar_proteins":4098}}}