{"metadata":{"accession":"PF19705","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR045776","hierarchy":null,"name":{"name":"PolF haem-oxygenase-like diiron oxidases","short":"PolF"},"description":[{"text":"<p>This entry represents PolF haem-oxygenase-like diiron oxidase which converts the desaturated l-isoleucine to polyoximic acid via an intramolecular C-N cyclization [[cite:PUB00164341]]. PolF exhibits dual functionality, orchestrating the sequential desaturation and cyclization with l-isoleucine as the initial substrate [[cite:PUB00164341]]. PolF folds into a seven-helix bundle, with three core helices coordinating the iron cofactor. Members of this family contain a highly conserved H-X3-E-X2-H sequence motif that completes the coordination of the diiron cofactors. A member belongs to a biosynthetic gene cluster (BGC). This BGC (BGC0000877) includes a member from the polyoxin A biosynthetic gene cluster from Streptomyces cacaoi subsp. asoensis [[cite:PUB00098985],[cite:PUB00098986]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00098986":{"PMID":19233844,"ISBN":null,"volume":"284","issue":"16","year":2009,"title":"Characterization of the polyoxin biosynthetic gene cluster from Streptomyces cacaoi and engineered production of polyoxin H.","URL":null,"raw_pages":"10627-38","medline_journal":"J Biol Chem","ISO_journal":"J Biol Chem","authors":["Chen W","Huang T","He X","Meng Q","You D","Bai L","Li J","Wu M","Li R","Xie Z","Zhou H","Zhou X","Tan H","Deng Z."],"DOI_URL":null},"PUB00098985":{"PMID":19383687,"ISBN":null,"volume":"155","issue":"Pt 6","year":2009,"title":"polR, a pathway-specific transcriptional regulatory gene, positively controls polyoxin biosynthesis in Streptomyces cacaoi subsp. asoensis.","URL":null,"raw_pages":"1819-1831","medline_journal":"Microbiology (Reading)","ISO_journal":"Microbiology (Reading)","authors":["Li R","Xie Z","Tian Y","Yang H","Chen W","You D","Liu G","Deng Z","Tan H."],"DOI_URL":null},"PUB00164341":{"PMID":41028918,"ISBN":null,"volume":null,"issue":null,"year":2025,"title":"A two-metalloenzyme cascade constructs the azetidine-containing pharmacophore.","URL":null,"raw_pages":null,"medline_journal":"Nat Chem","ISO_journal":"Nat Chem","authors":["Gong R","Qu Y","Liu J","Zhang X","Zhou L","Tian Z","Zeng X","Jin B","Li Z","Yu L","Chen R","Zhou Y","Liao L","Yang L","Song X","Cai YS","Shen K","Deng Z","Zhang Z","Wang B","Chen W."],"DOI_URL":"https://doi.org/10.1038/s41557-025-01949-y"}},"set_info":{"accession":"CL0230","name":"HO"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":1,"interactions":0,"matches":31,"pathways":0,"proteins":31,"proteomes":29,"sets":1,"structural_models":{"alphafold":14},"structures":11,"taxa":62},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":4,"alignment:full":11},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"9j2c","name":"Fe-SzPolF-L-isoleucine"}}}