{"metadata":{"accession":"PF19375","entry_id":null,"type":"family","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR045994","hierarchy":null,"name":{"name":"DurN substrate-assisted peptide maturase","short":"DurN"},"description":[{"text":"<p>This entry represents a member of a biosynthetic gene cluster (BGC) involved in duramycin biosynthesis, a ribosomally synthesized and post-translationally modified peptide (RiPP) produced by various actinomycetes and marine symbionts. Duramycin binds phosphatidylethanolamine (PE), a major structural phospholipid in mammalian and microbial cell membranes which contains 19 amino acids and 5 post-translational modifications (PTMs). This entry represents DurN, an enzyme that forms (2S,9S)-Lal one of the two last PTMs. DurN catalyses stereospecific and diastereoselective Lal formation in an unusual substrate-assisted mechanism in  which the active site residues that make the reaction proceed come from the substrate itself, manoeuvred into position by DurN [[cite:PUB00152101]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00152101":{"PMID":30177849,"ISBN":null,"volume":"14","issue":"10","year":2018,"title":"Substrate-assisted enzymatic formation of lysinoalanine in duramycin.","URL":null,"raw_pages":"928-933","medline_journal":"Nat Chem Biol","ISO_journal":"Nat Chem Biol","authors":["An L","Cogan DP","Navo CD","Jimenez-Oses G","Nair SK","van der Donk WA."],"DOI_URL":"https://doi.org/10.1038/s41589-018-0122-4"}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":1,"interactions":0,"matches":42,"pathways":0,"proteins":42,"proteomes":38,"sets":0,"structural_models":{"alphafold":23},"structures":3,"taxa":81},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":5,"alignment:full":15},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"6c0y","name":"Lysinoalanine synthase, DurN, from duramycin biosynthesis bound to duramycin"}}}