{"metadata":{"accession":"PF16473","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR033390","hierarchy":null,"name":{"name":"3'-5' exoribonuclease Rv2179c-like domain","short":"Rv2179c-like"},"description":[{"text":"<p>This is a highly divergent 3' exoribonuclease family. The proteins constitute a typical RNase fold, where the active site residues form a magnesium catalytic centre. The protein of the solved structure readily cleaves 3' overhangs in a time-dependent manner. It is similar to DEDD-type RNases and is an unusual ATP-binding protein that binds ATP and dATP. It forms a dimer in solution and both protomers in the asymmetric unit bind a magnesium ion through Asp-6 in SwissProt:P9WJ73 [[cite:PUB00074134]]. Proteins containing this domain also include 3'-5' exonuclease dexA from bacteriophage T4. It may play a role in the final step of host DNA degradation, by scavenging DNA into mononucleotides [[cite:PUB00078716],[cite:PUB00078717]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00074134":{"PMID":24311791,"ISBN":null,"volume":"289","issue":"4","year":2014,"title":"Mycobacterium tuberculosis Rv2179c protein establishes a new exoribonuclease family with broad phylogenetic distribution.","URL":null,"raw_pages":"2139-47","medline_journal":"J Biol Chem","ISO_journal":"J. Biol. Chem.","authors":["Abendroth J","Ollodart A","Andrews ES","Myler PJ","Staker BL","Edwards TE","Arcus VL","Grundner C."],"DOI_URL":"http://dx.doi.org/10.1074/jbc.M113.525683"},"PUB00078717":{"PMID":3056918,"ISBN":null,"volume":"170","issue":"12","year":1988,"title":"Effect of DNA sequence and structure on nuclease activity of the DexA protein of bacteriophage T4.","URL":null,"raw_pages":"5830-6","medline_journal":"J Bacteriol","ISO_journal":"J. Bacteriol.","authors":["Gruber H","Kern G","Gauss P","Gold L."],"DOI_URL":null},"PUB00078716":{"PMID":10581261,"ISBN":null,"volume":"153","issue":"4","year":1999,"title":"Role of exonucleolytic degradation in group I intron homing in phage T4.","URL":null,"raw_pages":"1501-12","medline_journal":"Genetics","ISO_journal":"Genetics","authors":["Huang YJ","Parker MM","Belfort M."],"DOI_URL":null}},"set_info":{"accession":"CL0219","name":"RNase_H"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":18,"interactions":0,"matches":2547,"pathways":0,"proteins":2547,"proteomes":2444,"sets":1,"structural_models":{"alphafold":1296},"structures":8,"taxa":4185},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":22,"alignment:full":1193},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"4oke","name":"Structure of RNase AS, a polyadenylate-specific exoribonuclease affecting mycobacterial virulence in vivo"}}}