{"metadata":{"accession":"PF12432","entry_id":null,"type":"repeat","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR022145","hierarchy":null,"name":{"name":"Integrator complex subunit 1, RP2B-binding domain","short":"INTS1_RP2B-bd"},"description":[{"text":"<p>Integrator (INT) complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. It binds the C-terminal domain (CTD) of RNA polymerase II (Pol II) and functions as an RNA endonuclease to cleave different classes of RNAs, regulating the transcription of both protein-coding genes and noncoding elements [[cite:PUB00152015],[cite:PUB00153137]]. Integrator also associates with protein phosphatase 2A (PP2A) core enzyme to form the INTAC complex [[cite:PUB00152015]] which regulates transcription through dephosphorylation of Pol II CTD. This entry includes the Integrator complex subunit 1 (INTS1) which forms the backbone module of the complex (it consists of four modules: backbone, shoulder, phosphatase and endonuclease) through the association with INTS2 and INTS7 to form a rigid scaffold. The backbone and shoulder modules form a central cruciform scaffold. INTS1 is an all-alpha helical protein with four repetitive helix hairpin domains towards the C-terminal (R1-R4) [[cite:PUB00152015]]. This entry represents a domain found at the N-terminal of INTS1, which interacts with Pol II subunit RPB2 and form interface A [[cite:PUB00153137]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00153137":{"PMID":34762484,"ISBN":null,"volume":"374","issue":"6569","year":2021,"title":"Structural basis of Integrator-mediated transcription regulation.","URL":null,"raw_pages":"883-887","medline_journal":"Science","ISO_journal":"Science","authors":["Fianu I","Chen Y","Dienemann C","Dybkov O","Linden A","Urlaub H","Cramer P."],"DOI_URL":"https://doi.org/10.1126/science.abk0154"},"PUB00152015":{"PMID":33243860,"ISBN":null,"volume":"370","issue":"6520","year":2020,"title":"Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase. ","URL":null,"raw_pages":"eabb5872","medline_journal":"Science","ISO_journal":"Science","authors":["Zheng H","Qi Y","Hu S","Cao X","Xu C","Yin Z","Chen X","Li Y","Liu W","Li J","Wang J","Wei G","Liang K","Chen FX","Xu Y."],"DOI_URL":"https://doi.org/10.1126/science.abb5872"}},"set_info":{"accession":"CL0020","name":"TPR"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":35,"interactions":0,"matches":1776,"pathways":0,"proteins":1741,"proteomes":1243,"sets":1,"structural_models":{"alphafold":141},"structures":8,"taxa":4101},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":19,"alignment:full":1369},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}