{"metadata":{"accession":"PF10986","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR021253","hierarchy":null,"name":{"name":"ZrgA family zinc uptake protein","short":"ZrgA"},"description":[{"text":"<p>This entry represents the ZrgA family of zinc uptake proteins. ZrgA plays a predominant role in zinc acquisition in Vibrio parahaemolyticus and other members of the Vibrionaceae family. The protein is part of the ZrgABCDE system that functions as a zinc transporter [[cite:PUB00159002]]. ZrgA expression is negatively regulated by the zinc uptake regulator (Zur) and is derepressed under zinc limitation conditions. The gene is part of an operon that includes zrgB (ABC transporter permease), zrgC (ABC transporter ATP-binding protein), zrgD and zrgE. The ZrgA protein contains several conserved motifs potentially involved in zinc binding. The wild type ZrgA can bind up to 10 zinc ions with four high-affinity zinc binding sites [[cite:PUB00159005]]. The structures of ZrgA from Vibrio cholerae and its homologue, protein PA4063 from Pseudomonas aeruginosa, were determined bound to zinc [[cite:PUB00159005],[cite:PUB00159006]]. These proteins adopt an alpha/beta structure consisting of a six-stranded meander beta-sheet and two alpha-helices packed on it with an unique topology.</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00159005":{"PMID":36613986,"ISBN":null,"volume":"24","issue":"1","year":2022,"title":"Structure and Function of the Zinc Binding Protein ZrgA from <i>Vibrio cholerae</i>.","URL":null,"raw_pages":"548","medline_journal":"Int J Mol Sci","ISO_journal":"Int J Mol Sci","authors":["Valencia DW","Melendez AB","Melendrez IA","Yukl ET."],"DOI_URL":"https://doi.org/10.3390/ijms24010548"},"PUB00159002":{"PMID":36482737,"ISBN":null,"volume":"14","issue":"1","year":2023,"title":"ZrgA contributes to zinc acquisition in Vibrio parahaemolyticus.","URL":null,"raw_pages":"2156196","medline_journal":"Virulence","ISO_journal":"Virulence","authors":["Zheng C","Qiu J","Zhai Y","Wei M","Zhou X","Jiao X."],"DOI_URL":"https://doi.org/10.1080/21505594.2022.2156196"},"PUB00159006":{"PMID":34726168,"ISBN":null,"volume":"77","issue":"Pt 11","year":2021,"title":"Structure and metal-binding properties of PA4063, a novel player in periplasmic zinc trafficking by Pseudomonas aeruginosa.","URL":null,"raw_pages":"1401-1410","medline_journal":"Acta Crystallogr D Struct Biol","ISO_journal":"Acta Crystallogr D Struct Biol","authors":["Fiorillo A","Battistoni A","Ammendola S","Secli V","Rinaldo S","Cutruzzola F","Demitri N","Ilari A."],"DOI_URL":"https://doi.org/10.1107/S2059798321009608"}},"set_info":{"accession":"CL0966","name":"ZrgA-like"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":5,"interactions":0,"matches":1522,"pathways":0,"proteins":1443,"proteomes":1386,"sets":1,"structural_models":{"alphafold":869},"structures":6,"taxa":2045},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":388,"alignment:full":698},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"8ezw","name":"Structure of Apo ZrgA deletion 124-184 from Vibrio cholerae"}}}