This conserved domain is found in Thyroid adenoma-associated proteins (THADA) and the yeast homologue, TRM732. Trm732 forms a complex with the methyltransferase Trm7 to 2'- O-methylate tRNA residue 32 (Nm32), being required for Trm7 methylation activity. In humans, mutations of the Trm7 homologue FTSJ1, which interacts with THADA, impair Nm32 modifications, associated with non-syndromic X-linked intellectual disability [[cite:PUB00098320],[cite:PUB00098321],[cite:PUB00098322]]. It has been suggested that these proteins may play a role in additional biological processes not related to translation. This domain contains a RRSAGLP conserved motif that is required for tRNA modification activity (Funk HM et.al., Preprint from bioRxiv, 03 Jun 2021 DOI: 10.1101/2021.06.03.446962) [[cite:PUB00098320],[cite:PUB00098321],[cite:PUB00098322]].
","llm":false,"checked":false,"updated":false}],"wikipedia":[{"title":"Domain_of_unknown_function","extract":"A domain of unknown function (DUF) is a protein domain that has no characterised function. These families have been collected together in the Pfam database using the prefix DUF followed by a number, with examples being DUF2992 and DUF1220. As of 2019, there are almost 4,000 DUF families within the Pfam database representing over 22% of known families. Some DUFs are not named using the nomenclature due to popular usage but are nevertheless DUFs.
","thumbnail":null}],"literature":{"PUB00098321":{"PMID":25625329,"ISBN":null,"volume":"11","issue":"12","year":2014,"title":"Two-subunit enzymes involved in eukaryotic post-transcriptional tRNA modification.","URL":null,"raw_pages":"1608-18","medline_journal":"RNA Biol","ISO_journal":"RNA Biol","authors":["Guy MP","Phizicky EM."],"DOI_URL":null},"PUB00098320":{"PMID":31586407,"ISBN":null,"volume":"47","issue":"20","year":2019,"title":"Structure of tRNA methyltransferase complex of Trm7 and Trm734 reveals a novel binding interface for tRNA recognition.","URL":null,"raw_pages":"10942-10955","medline_journal":"Nucleic Acids Res","ISO_journal":"Nucleic Acids Res","authors":["Hirata A","Okada K","Yoshii K","Shiraishi H","Saijo S","Yonezawa K","Shimizu N","Hori H."],"DOI_URL":null},"PUB00098322":{"PMID":25404562,"ISBN":null,"volume":"21","issue":"1","year":2015,"title":"Conservation of an intricate circuit for crucial modifications of the tRNAPhe anticodon loop in eukaryotes.","URL":null,"raw_pages":"61-74","medline_journal":"RNA","ISO_journal":"RNA","authors":["Guy MP","Phizicky EM."],"DOI_URL":null}},"set_info":{"accession":"CL0020","name":"TPR"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":87,"interactions":0,"matches":4613,"pathways":0,"proteins":4556,"proteomes":3000,"sets":1,"structural_models":{"alphafold":662},"structures":1,"taxa":7989},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":124,"alignment:full":3466},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}