{"metadata":{"accession":"PF10288","entry_id":null,"type":"family","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR019407","hierarchy":null,"name":{"name":"Cytoplasmic tRNA 2-thiolation protein 2","short":"CTU2"},"description":[{"text":"<p>CTU2 is a family of proteins necessary for the formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. The family is conserved from plants to humans ]1]. It plays a central role in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine, or the wobble nucleoside [[cite:PUB00109223]]. This wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U), is required for the proper decoding of NNR codons in eukaryotes. The 2-thio group gives rigidity by largely fixing the C3'-endo ribose puckering, ensuring stable and accurate codon-anticodon pairing [[cite:PUB00069587]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00045305":{"PMID":18391219,"ISBN":null,"volume":"105","issue":"14","year":2008,"title":"The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity.","URL":null,"raw_pages":"5459-64","medline_journal":"Proc Natl Acad Sci U S A","ISO_journal":"Proc. Natl. Acad. Sci. U.S.A.","authors":["Dewez M","Bauer F","Dieu M","Raes M","Vandenhaute J","Hermand D."],"DOI_URL":"http://dx.doi.org/10.1073/pnas.0709404105"},"PUB00069587":{"PMID":19151091,"ISBN":null,"volume":"37","issue":"4","year":2009,"title":"Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions.","URL":null,"raw_pages":"1335-52","medline_journal":"Nucleic Acids Res","ISO_journal":"Nucleic Acids Res.","authors":["Noma A","Sakaguchi Y","Suzuki T."],"DOI_URL":"http://dx.doi.org/10.1093/nar/gkn1023"},"PUB00109223":{"PMID":18755837,"ISBN":null,"volume":"14","issue":"10","year":2008,"title":"A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae.","URL":null,"raw_pages":"2183-94","medline_journal":"RNA","ISO_journal":"RNA","authors":["Huang B","Lu J","Bystrom AS."],"DOI_URL":null}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":39,"interactions":0,"matches":2997,"pathways":0,"proteins":2997,"proteomes":2511,"sets":0,"structural_models":{"alphafold":2543},"structures":0,"taxa":6765},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":95,"alignment:full":2301},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}