{"metadata":{"accession":"PF09992","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR018711","hierarchy":null,"name":{"name":"Phosphodiester glycosidase","short":"NAGPA"},"description":[{"text":"<p>This is a family conserved from bacteria to humans. The structure of a member from Bacteroides has been crystallised and modelled onto the luminal region of the human member of the family, the transmembrane glycoprotein N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase. There is some conservation of potentially functional residues, implying that in the bacterial members this family acts in some way as a phosphodiester glycosidase [[cite:PUB00078675]]. The human protein is also present, so the eukaryotic members are likely to be catalysing the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides [[cite:PUB00108993]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00108993":{"PMID":20147709,"ISBN":null,"volume":"362","issue":"8","year":2010,"title":"Mutations in the lysosomal enzyme-targeting pathway and persistent stuttering.","URL":null,"raw_pages":"677-85","medline_journal":"N Engl J Med","ISO_journal":"N Engl J Med","authors":["Kang C","Riazuddin S","Mundorff J","Krasnewich D","Friedman P","Mullikin JC","Drayna D."],"DOI_URL":null},"PUB00078675":{"PMID":23572527,"ISBN":null,"volume":"288","issue":"23","year":2013,"title":"Structure and function of the DUF2233 domain in bacteria and in the human mannose 6-phosphate uncovering enzyme.","URL":null,"raw_pages":"16789-99","medline_journal":"J Biol Chem","ISO_journal":"J. Biol. Chem.","authors":["Das D","Lee WS","Grant JC","Chiu HJ","Farr CL","Vance J","Klock HE","Knuth MW","Miller MD","Elsliger MA","Deacon AM","Godzik A","Lesley SA","Kornfeld S","Wilson IA."],"DOI_URL":"http://dx.doi.org/10.1074/jbc.M112.434977"}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":247,"interactions":0,"matches":11569,"pathways":0,"proteins":11558,"proteomes":7460,"sets":0,"structural_models":{"alphafold":7114},"structures":9,"taxa":12626},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":106,"alignment:full":6513},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"3ohg","name":"Crystal structure of a protein with unknown function from DUF2233 family (BACOVA_00430) from Bacteroides ovatus at 1.80 A resolution"}}}