{"metadata":{"accession":"PF09369","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR018973","hierarchy":null,"name":{"name":"MrfA Zn-binding domain","short":"MZB"},"description":[{"text":"<p>This is the C-terminal MrfA Zn+2-binding domain (MZB, also referred to as DUF1998) which contains a conserved four-cysteine signature motif.  These four Cys reside in a short coil between two alpha-helices and form a metal ion-binding site [[cite:PUB00098193]]. This domain is frequently found at the C-terminal of ndNTPases, however, it is also found encoded in a standalone gene, downstream of putative helicase domain-encoding genes associated with bacterial anti-phage defense system DISARM. MrfA (Mitomycin repair factor A, also known as YprA in Bacillus subtilis) is a DNA helicase that supports repair of mitomycin C-induced DNA damage. MrfA homologues are widely distributed in bacteria and are also present in archaea, fungi and plants. The MrfA-homologue in yeast, Hrq1, also reduces mitomycin C sensitivity. Hrq1 has high similarity to human RecQ4 and was therefore assigned to the RecQ-like helicase family. MrfA homologues appear to be missing in Enterobacteria, however, certain pathogenic Escherichia coli and Salmonella strains harbour Z5898-like helicases with this domain [[cite:PUB00098193]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00098193":{"PMID":33300032,"ISBN":null,"volume":"49","issue":"1","year":2021,"title":"A skipping rope translocation mechanism in a widespread family of DNA repair helicases.","URL":null,"raw_pages":"504-518","medline_journal":"Nucleic Acids Res","ISO_journal":"Nucleic Acids Res","authors":["Roske JJ","Liu S","Loll B","Neu U","Wahl MC."],"DOI_URL":null}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":99,"interactions":0,"matches":11161,"pathways":0,"proteins":11160,"proteomes":8342,"sets":0,"structural_models":{"alphafold":5747},"structures":5,"taxa":13990},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":323,"alignment:full":6148},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}