{"metadata":{"accession":"PF09170","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR015253","hierarchy":null,"name":{"name":"CST, complex subunit STN1, C terminal","short":"STN1_2"},"description":[{"text":"<p>STN1 is a component of the CST complex, a complex that binds to single-stranded DNA and is required for protecting telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity on their own. In addition to telomere protection, the CST complex probably has a more general role in DNA metabolism at non-telomeric sites. This entry represents the C-terminal region of Stn1 which has two winged helix-turn-helix (wHTH) motifs, wHTH1 and wHTH2 [[cite:PUB00053885],[cite:PUB00097419]]. wHTH1 is structurally similar to that in RPA32 with a large insertion between helices alpha2 and alpha3, unique to Stn1, that may allow interaction with a different set of proteins that function at telomeres such as Ctc1 [[cite:PUB00097419]]. wHTH2 is most similar to the DNA-binding wHTH motifs of the pur operon repressor and RepE replication initiator, but it does not bind double-stranded DNA [[cite:PUB00057805]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00057805":{"PMID":20008938,"ISBN":null,"volume":"23","issue":"24","year":2009,"title":"Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres.","URL":null,"raw_pages":"2900-14","medline_journal":"Genes Dev","ISO_journal":"Genes Dev.","authors":["Sun J","Yu EY","Yang Y","Confer LA","Sun SH","Wan K","Lue NF","Lei M."],"DOI_URL":"http://dx.doi.org/10.1101/gad.1851909"},"PUB00053884":{"PMID":19854130,"ISBN":null,"volume":"36","issue":"2","year":2009,"title":"RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway.","URL":null,"raw_pages":"193-206","medline_journal":"Mol Cell","ISO_journal":"Mol. Cell","authors":["Miyake Y","Nakamura M","Nabetani A","Shimamura S","Tamura M","Yonehara S","Saito M","Ishikawa F."],"DOI_URL":"http://dx.doi.org/10.1016/j.molcel.2009.08.009"},"PUB00097419":{"PMID":23826127,"ISBN":null,"volume":"8","issue":"6","year":2013,"title":"Structure of the human telomeric Stn1-Ten1 capping complex.","URL":null,"raw_pages":"e66756","medline_journal":"PLoS One","ISO_journal":"PLoS One","authors":["Bryan C","Rice C","Harkisheimer M","Schultz DC","Skordalakes E."],"DOI_URL":null},"PUB00152258":{"PMID":19119139,"ISBN":null,"volume":"284","issue":"9","year":2009,"title":"A DNA polymerase-{alpha}{middle dot}primase cofactor with homology to replication protein A-32 regulates DNA replication in mammalian cells.","URL":null,"raw_pages":"5807-18","medline_journal":"J Biol Chem","ISO_journal":"J Biol Chem","authors":["Casteel DE","Zhuang S","Zeng Y","Perrino FW","Boss GR","Goulian M","Pilz RB."],"DOI_URL":"https://doi.org/10.1074/jbc.M807593200"},"PUB00053885":{"PMID":19648609,"ISBN":null,"volume":"284","issue":"39","year":2009,"title":"OB fold-containing protein 1 (OBFC1), a human homolog of yeast Stn1, associates with TPP1 and is implicated in telomere length regulation.","URL":null,"raw_pages":"26725-31","medline_journal":"J Biol Chem","ISO_journal":"J. Biol. Chem.","authors":["Wan M","Qin J","Songyang Z","Liu D."],"DOI_URL":"http://dx.doi.org/10.1074/jbc.M109.021105"}},"set_info":{"accession":"CL0123","name":"HTH"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":13,"interactions":0,"matches":1210,"pathways":0,"proteins":1178,"proteomes":755,"sets":1,"structural_models":{"alphafold":1016},"structures":8,"taxa":2397},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":19,"alignment:full":988},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"4jqf","name":"Structure of the C-terminal domain of human telomeric Stn1"}}}