{"metadata":{"accession":"PF07510","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR011089","hierarchy":null,"name":{"name":"GmrSD restriction endonucleases, C-terminal domain","short":"GmrSD_C"},"description":[{"text":"<p>This entry (previously known as DUF1524) represents a domain found C-terminal in the GmrSD family of modification-dependent restriction endonucleases including SspE proteins [[cite:PUB00155638]]. SspE is a dual-function protein that exerts both N-terminal NTPase and C-terminal DNA nicking nuclease activities to defend against phage infection [[cite:PUB00155272]]. SspE has a phosphorothioate (PT) modification-binding preference and utilises epigenetic PT modification to accomplish self-nonself discrimination and to coordinate its NTPase and DNA nicking activities to fight against phages. The domain represented in this entry has a DNA nicking nuclease activity and belongs to HNH nuclease superfamily.</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00155638":{"PMID":26493560,"ISBN":null,"volume":"16","issue":null,"year":2015,"title":"Phylogenomics and sequence-structure-function relationships in the GmrSD family of Type IV restriction enzymes.","URL":null,"raw_pages":"336","medline_journal":"BMC Bioinformatics","ISO_journal":"BMC Bioinformatics","authors":["Machnicka MA","Kaminska KH","Dunin-Horkawicz S","Bujnicki JM."],"DOI_URL":"https://doi.org/10.1186/s12859-015-0773-z"},"PUB00155272":{"PMID":36351933,"ISBN":null,"volume":"13","issue":"1","year":2022,"title":"Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE.","URL":null,"raw_pages":"6773","medline_journal":"Nat Commun","ISO_journal":"Nat Commun","authors":["Gao H","Gong X","Zhou J","Zhang Y","Duan J","Wei Y","Chen L","Deng Z","Wang J","Chen S","Wu G","Wang L."],"DOI_URL":"https://doi.org/10.1038/s41467-022-34505-0"}},"set_info":{"accession":"CL0263","name":"His-Me_finger"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":95,"interactions":0,"matches":13171,"pathways":0,"proteins":13168,"proteomes":7699,"sets":1,"structural_models":{"alphafold":7977},"structures":7,"taxa":11960},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":57,"alignment:full":6596},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}