{"metadata":{"accession":"PF07314","entry_id":null,"type":"family","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR010178","hierarchy":null,"name":{"name":"Lipoprotein intramolecular transacylase Lit","short":"Lit"},"description":[{"text":"<p>This entry represents the lipoprotein intramolecular transacylase, Lit [[cite:PUB00158976],[cite:PUB00158977]]. Lit creates a lipoprotein that is less immunogenic, possibly enabling the bacteria to gain a foothold in the host by stealth. The crystal structure of the Lit enzyme from Bacillus cereus ([swissprot:Q813T3]) revealed that it consists of consists of four transmembrane helices with an extracellular cap [[cite:PUB00158977]]. The conserved residues map to the cap-membrane interface and they include two catalytic histidines that function to effect unimolecular transacylation. The reaction involves acyl transfer from the sn-2 position of the glyceryl moiety to the amino group on the N-terminal cysteine of the substrate via an 8-membered ring intermediate.</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00158976":{"PMID":28320885,"ISBN":null,"volume":"199","issue":"11","year":2017,"title":"Identification of the Lyso-Form <i>N</i>-Acyl Intramolecular Transferase in Low-GC Firmicutes.","URL":null,"raw_pages":"e00099-17","medline_journal":"J Bacteriol","ISO_journal":"J Bacteriol","authors":["Armbruster KM","Meredith TC."],"DOI_URL":"https://doi.org/10.1128/JB.00099-17"},"PUB00158977":{"PMID":34253723,"ISBN":null,"volume":"12","issue":"1","year":2021,"title":"Structural basis of the membrane intramolecular transacylase reaction responsible for lyso-form lipoprotein synthesis.","URL":null,"raw_pages":"4254","medline_journal":"Nat Commun","ISO_journal":"Nat Commun","authors":["Olatunji S","Bowen K","Huang CY","Weichert D","Singh W","Tikhonova IG","Scanlan EM","Olieric V","Caffrey M."],"DOI_URL":"https://doi.org/10.1038/s41467-021-24475-0"}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":4,"interactions":0,"matches":1709,"pathways":0,"proteins":1709,"proteomes":1655,"sets":0,"structural_models":{"alphafold":1127},"structures":4,"taxa":2669},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":53,"alignment:full":933},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"7b0p","name":"In meso structure of the membrane integral lipoprotein intramolecular transacylase Lit from Bacillus cereus in space group P21212"}}}