{"metadata":{"accession":"PF06664","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR047843","hierarchy":null,"name":{"name":"Wntless-like, transmembrane domain","short":"WLS-like_TM"},"description":[{"text":"<p>This entry represents the seven transmembrane domain of wntless (WLS) from Drosophila and its homologues, also known as MIG-14 in C.elegans which regulates endocytosis and secretion of the wnt ligand egl-20[[cite:PUB00058252]]. Newly synthesised EGL-20/Wnt binds to MIG-14 in the Golgi, targetting the Wnt to the cell membrane for secretion. AP-2-mediated endocytosis and retromer retrieval at the sorting endosome would recycle MIG-14 to the Golgi, where it can bind to EGL-20/Wnt for next cycle of secretion [[cite:PUB00058252],[cite:PUB00154330]]. WLS is a membrane trafficking and secretion chaperone for lipidated Wnt signaling proteins [[cite:PUB00151072]]. It regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism [[cite:PUB00151072],[cite:PUB00061859]]. This entry also includes TMEM181, a cell surface protein associated with Escherichia coli CDT toxicity [[cite:PUB00151072]]. Members of this entry are homologous to GPR180 and TMEM145, and together with TMEM87A/B, GPR107 and GPR108, they have been described as GOLD domain seven-transmembrane helix (GOST) proteins given their domain architecture similarities. They all consist of a GOLD domain atop of a seven-transmembrane helix domain [[cite:PUB00151072]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00154330":{"PMID":18160347,"ISBN":null,"volume":"14","issue":"1","year":2008,"title":"Wnt signaling requires retromer-dependent recycling of MIG-14/Wntless in Wnt-producing cells.","URL":null,"raw_pages":"140-7","medline_journal":"Dev Cell","ISO_journal":"Dev Cell","authors":["Yang PT","Lorenowicz MJ","Silhankova M","Coudreuse DY","Betist MC","Korswagen HC."],"DOI_URL":"https://doi.org/10.1016/j.devcel.2007.12.004"},"PUB00061859":{"PMID":16678095,"ISBN":null,"volume":"125","issue":"3","year":2006,"title":"Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells.","URL":null,"raw_pages":"509-22","medline_journal":"Cell","ISO_journal":"Cell","authors":["Banziger C","Soldini D","Schutt C","Zipperlen P","Hausmann G","Basler K."],"DOI_URL":"http://dx.doi.org/10.1016/j.cell.2006.02.049"},"PUB00058252":{"PMID":18160346,"ISBN":null,"volume":"14","issue":"1","year":2008,"title":"C. elegans AP-2 and retromer control Wnt signaling by regulating mig-14/Wntless.","URL":null,"raw_pages":"132-9","medline_journal":"Dev Cell","ISO_journal":"Dev. Cell","authors":["Pan CL","Baum PD","Gu M","Jorgensen EM","Clark SG","Garriga G."],"DOI_URL":"http://dx.doi.org/10.1016/j.devcel.2007.12.001"},"PUB00151072":{"PMID":36373655,"ISBN":null,"volume":"11","issue":null,"year":2022,"title":"Structure of the GOLD-domain seven-transmembrane helix protein family member TMEM87A.","URL":null,"raw_pages":"e81704","medline_journal":"Elife","ISO_journal":"Elife","authors":["Hoel CM","Zhang L","Brohawn SG."],"DOI_URL":null}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":54,"interactions":0,"matches":3913,"pathways":0,"proteins":3813,"proteomes":1345,"sets":0,"structural_models":{"alphafold":3297},"structures":9,"taxa":4379},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":51,"alignment:full":2964},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}