{"metadata":{"accession":"PF06581","entry_id":null,"type":"family","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR009511","hierarchy":null,"name":{"name":"Mad1 and Cdc20-bound-Mad2 binding","short":"p31comet"},"description":[{"text":"<p>This family is involved in the cell-cycle surveillance mechanism called the spindle checkpoint. This mechanism monitors the proper bipolar attachment of sister chromatids to spindle microtubules and ensures the fidelity of chromosome segregation during mitosis. A key player in mitosis is Mad2, and Mad2 exhibits an unusual two-state behaviour. A Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerisation and converts Mad2 to a conformer amenable to Cdc20 binding. p31comet inactivates the checkpoint by binding to Mad1- or Cdc20-bound Mad2 in such a way as to stop Mad2 activation and to promote the dissociation of the Mad2-Cdc20 complex [[cite:PUB00049186]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00049186":{"PMID":18022368,"ISBN":null,"volume":"131","issue":"4","year":2007,"title":"p31comet blocks Mad2 activation through structural mimicry.","URL":null,"raw_pages":"744-55","medline_journal":"Cell","ISO_journal":"Cell","authors":["Yang M","Li B","Tomchick DR","Machius M","Rizo J","Yu H","Luo X."],"DOI_URL":"http://dx.doi.org/10.1016/j.cell.2007.08.048"}},"set_info":{"accession":"CL0651","name":"Mad2"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":7,"interactions":0,"matches":1201,"pathways":0,"proteins":1184,"proteomes":998,"sets":1,"structural_models":{"alphafold":1041},"structures":2,"taxa":3345},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":4,"alignment:full":926},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}