{"metadata":{"accession":"PF05251","entry_id":null,"type":"family","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR007915","hierarchy":null,"name":{"name":"Oligosaccharyltransferase subunit 5","short":"Ost5"},"description":[{"text":"<p>Eukaryotic N-glycosylation is catalysed in the ER lumen, where the enzyme oligosaccharyltransferase (OTase) transfers donor glycans from a dolichol pyrophosphate (DolP) carrier (Lipid-linked oligosaccharide; LLO) to polypeptides. The yeast OTase is a hetero-oligomeric complex composed of essential (Ost1, Ost2, Wbp1, Stt3, and Swp1) and nonessential (Ost3, Ost4, Ost5, and Ost6) subunits. This domain family is found in Ost5, [swissprot:Q92316]. The precise function of this subunit is not known, however Ost5 appears to form a sub-complex with Ost1, and this sub-complex associates with the catalytic Stt3 subunit of OTase. Down regulation of Ost5 resulted in a limited effect on glycosylation and no effect on the stability of Ost1 or Stt3 subunits [[cite:PUB00109240],[cite:PUB00109241]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00109240":{"PMID":27094473,"ISBN":null,"volume":"15","issue":"7","year":2016,"title":"SWATH-MS Glycoproteomics Reveals Consequences of Defects in the Glycosylation Machinery.","URL":null,"raw_pages":"2435-47","medline_journal":"Mol Cell Proteomics","ISO_journal":"Mol Cell Proteomics","authors":["Zacchi LF","Schulz BL."],"DOI_URL":null},"PUB00109241":{"PMID":9135133,"ISBN":null,"volume":"16","issue":"6","year":1997,"title":"A specific screen for oligosaccharyltransferase mutations identifies the 9 kDa OST5 protein required for optimal activity in vivo and in vitro.","URL":null,"raw_pages":"1164-72","medline_journal":"EMBO J","ISO_journal":"EMBO J","authors":["Reiss G","te Heesen S","Gilmore R","Zufferey R","Aebi M."],"DOI_URL":null}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":59,"interactions":0,"matches":3052,"pathways":0,"proteins":3042,"proteomes":2536,"sets":0,"structural_models":{"alphafold":2628},"structures":12,"taxa":7096},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":148,"alignment:full":2340},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}