{"metadata":{"accession":"PF05168","entry_id":null,"type":"domain","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR007842","hierarchy":null,"name":{"name":"HEPN domain","short":"HEPN"},"description":null,"wikipedia":[{"title":"HEPN_domain","extract":"<p>In molecular biology, the <b>HEPN domain</b> is a region of approximately 110 amino acids found in the C terminus of sacsin, a chaperonin implicated in an early-onset neurodegenerative disease in human, and in many bacterial and archaea proteins. There are three classes of proteins with HEPN domains:</p><ul><li>Single-domain HEPN proteins found in many bacteria.</li>\n<li>Two-domain proteins with N-terminal nucleotidyltransferase (NT) and C- terminal HEPN domains. This N-terminal NT domain belongs to a large family of NTs, which includes several classes of enzymes that are responsible for some types of bacterial resistance to aminoglycosides. These enzymes deactivate various antibiotics by transferring a nucleotidyl group to the drug.</li>\n<li>A multidomain sacsin protein in genomes of fish and mammals. The HEPN domain is located at the C terminus of the protein, directly after the DnaJ domain. The crystal structure of the HEPN domain from the TM0613 protein of <i>Thermotoga maritima</i> indicates that it is structurally similar to the C-terminal all-alpha-helical domain of kanamycin nucleotidyltransferases (KNTases). It is composed of five alpha helices, three of which form an up- and-down helical bundle, with a pair of short helices on the side. The distant structural similarity suggests that the HEPN domain might be involved in nucleotide binding.</li></ul>","thumbnail":null}],"literature":null,"set_info":{"accession":"CL0291","name":"KNTase_C"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":50,"interactions":0,"matches":7296,"pathways":0,"proteins":7290,"proteomes":3476,"sets":1,"structural_models":{"alphafold":4256},"structures":6,"taxa":7657},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":76,"alignment:full":4596},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"1ufb","name":"Crystal structure of TT1696 from Thermus thermophilus HB8"}}}