{"metadata":{"accession":"PF04751","entry_id":null,"type":"family","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR006839","hierarchy":null,"name":{"name":"Dual-action ribosomal maturation protein DarP","short":"DarP"},"description":[{"text":"<p>The entry, previously known as DUF615, represents the Dual-action ribosomal maturation protein DarP (also known as YjgA) from Escherichia coli and related proteins. This protein binds pre-50S ribosomal subunits near the 23S rRNA L1 stalk, where it probably mediate correct positioning of the L1 stalk [[cite:PUB00104166]]. It plays a dual role in the late stages of assembly of the 50S ribosomal subunit where it promotes peptidyl transferase center maturation by modulating the docking of 23S rRNA helix H68, and maintains the stability of helix H89 with the help of uL16 (rplP), facilitating the final maturation of the peptidyl transferase center [[cite:PUB00155442]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00104166":{"PMID":33639093,"ISBN":null,"volume":"81","issue":"6","year":2021,"title":"Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization.","URL":null,"raw_pages":"1200-1215.e9","medline_journal":"Mol Cell","ISO_journal":"Mol Cell","authors":["Nikolay R","Hilal T","Schmidt S","Qin B","Schwefel D","Vieira-Vieira CH","Mielke T","Burger J","Loerke J","Amikura K","Flugel T","Ueda T","Selbach M","Deuerling E","Spahn CMT."],"DOI_URL":null},"PUB00155442":{"PMID":38842932,"ISBN":null,"volume":"52","issue":"13","year":2024,"title":"YjgA plays dual roles in enhancing PTC maturation.","URL":null,"raw_pages":"7947-7960","medline_journal":"Nucleic Acids Res","ISO_journal":"Nucleic Acids Res","authors":["Du M","Deng C","Yu T","Zhou Q","Zeng F."],"DOI_URL":"https://doi.org/10.1093/nar/gkae469"}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":22,"interactions":0,"matches":4919,"pathways":0,"proteins":4911,"proteomes":4586,"sets":0,"structural_models":{"alphafold":3314},"structures":5,"taxa":6784},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":109,"alignment:full":2105},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}