{"metadata":{"accession":"PF01969","entry_id":null,"type":"family","go_terms":null,"source_database":"pfam","member_databases":null,"integrated":"IPR002822","hierarchy":null,"name":{"name":"Nickel insertion protein","short":"Ni_insertion"},"description":[{"text":"<p>Members of this family may be involved in the activation of nickel-pincer cofactor-dependent enzymes. LarC from Lactobacillus plantarum is involved, together with LarB and LarE, in the synthesis of the enzyme-bound cofactor of lactate racemase (LarA). Larc C binds Ni2+, and functions in nickel delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN), to form the mature cofactor [[cite:PUB00085157],[cite:PUB00086644]].</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00086644":{"PMID":27114550,"ISBN":null,"volume":"113","issue":"20","year":2016,"title":"Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion.","URL":null,"raw_pages":"5598-603","medline_journal":"Proc Natl Acad Sci U S A","ISO_journal":"Proc. Natl. Acad. Sci. U.S.A.","authors":["Desguin B","Soumillion P","Hols P","Hausinger RP."],"DOI_URL":"https://doi.org/10.1073/pnas.1600486113"},"PUB00085157":{"PMID":24710389,"ISBN":null,"volume":"5","issue":null,"year":2014,"title":"Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.","URL":null,"raw_pages":"3615","medline_journal":"Nat Commun","ISO_journal":"Nat Commun","authors":["Desguin B","Goffin P","Viaene E","Kleerebezem M","Martin-Diaconescu V","Maroney MJ","Declercq JP","Soumillion P","Hols P."],"DOI_URL":"https://doi.org/10.1038/ncomms4615"}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":19,"interactions":0,"matches":4175,"pathways":0,"proteins":4090,"proteomes":3294,"sets":0,"structural_models":{"alphafold":2928},"structures":5,"taxa":6143},"entry_annotations":{"hmm":0,"logo":0,"alignment:seed":212,"alignment:full":2593},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":{"accession":"6bwr","name":"LarC2, the C-terminal domain of a cyclometallase involved in the synthesis of the NPN cofactor of lactate racemase, in complex with nickel"}}}