{"metadata":{"accession":"cd09467","entry_id":null,"type":"domain","go_terms":null,"source_database":"cdd","member_databases":null,"integrated":"IPR049593","hierarchy":null,"name":{"name":"The first LIM domain of Lhx3b","short":"LIM1_Lhx3b"},"description":[{"text":"<p>The first LIM domain of Lhx3b. Lhx3b is a member of LHX protein family, which features two tandem N-terminal LIM domains and a C-terminal DNA binding homeodomain. Members of LHX family are found in the nucleus and act as transcription factors or cofactors. LHX proteins are critical for the development of specialized cells in multiple tissue types, including the nervous system, skeletal muscle, the heart, the kidneys, and endocrine organs, such as the pituitary gland and the pancreas. Lhx3b is one of the two isoforms of Lhx3. The Lhx3 gene is expressed in the ventral spinal cord, the pons, the medulla oblongata, and the pineal gland of the developing nervous system during mouse embryogenesis, and transcripts are found in the emergent pituitary gland. Lhx3 functions in concert with other transcription factors to specify interneuron and motor neuron fates during development. Lhx3 proteins have been demonstrated to directly bind to the promoters of several pituitary hormone gene promoters. The Lhx3 gene encodes two isoforms, LHX3a and LHX3b that differ in their amino-terminal sequences, where Lhx3a has longer N-terminal.  They show differential activation of pituitary hormone genes and distinct DNA binding properties. In human, Lhx3a trans-activated the alpha-glycoprotein subunit promoter and genes containing a high-affinity Lhx3 binding site more effectively than the hLhx3b isoform. In addition, hLhx3a induce transcription of the TSHbeta-subunit gene by acting on pituitary POU domain factor, Pit-1, while hLhx3b does not. As in other LIM domains, this domain family is 50-60 amino acids in size and shares two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assembly of multimeric protein. [[cite:PUB00125080], [cite:PUB00125079], [cite:PUB00125081], [cite:PUB00125078], [cite:PUB00007267], [cite:PUB00124086], [cite:PUB00093154], [cite:PUB00071839], [cite:PUB00080208], [cite:PUB00007268], [cite:PUB00080209], [cite:PUB00080191], [cite:PUB00080210], [cite:PUB00080211], [cite:PUB00080212], [cite:PUB00005529], [cite:PUB00125082], [cite:PUB00124058], [cite:PUB00124088], [cite:PUB00124087]]</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00005529":{"PMID":7974745,"ISBN":null,"volume":"10","issue":"9","year":1994,"title":"The LIM domain: a new structural motif found in zinc-finger-like proteins.","URL":null,"raw_pages":"315-20","medline_journal":"Trends Genet","ISO_journal":"Trends Genet.","authors":["Sanchez-Garcia I","Rabbitts TH."],"DOI_URL":"http://dx.doi.org/10.1016/0168-9525(94)90034-5"},"PUB00080212":{"PMID":9190203,"ISBN":null,"volume":"8","issue":"2","year":1997,"title":"Molecular dissection of a LIM domain.","URL":null,"raw_pages":"219-30","medline_journal":"Mol Biol Cell","ISO_journal":"Mol. Biol. Cell","authors":["Schmeichel KL","Beckerle MC."],"DOI_URL":"http://dx.doi.org/10.1091/mbc.8.2.219"},"PUB00125079":{"PMID":15865204,"ISBN":null,"volume":"32","issue":"1","year":2005,"title":"DNA recognition properties of the LHX3b LIM homeodomain transcription factor.","URL":null,"raw_pages":"1-6","medline_journal":"Mol Biol Rep","ISO_journal":"Mol Biol Rep","authors":["Yaden BC","Savage JJ","Hunter CS","Rhodes SJ."],"DOI_URL":null},"PUB00125080":{"PMID":17210222,"ISBN":null,"volume":"265-266","issue":null,"year":2007,"title":"Roles of the LHX3 and LHX4 LIM-homeodomain factors in pituitary development.","URL":null,"raw_pages":"190-5","medline_journal":"Mol Cell Endocrinol","ISO_journal":"Mol Cell Endocrinol","authors":["Mullen RD","Colvin SC","Hunter CS","Savage JJ","Walvoord EC","Bhangoo AP","Ten S","Weigel J","Pfaffle RW","Rhodes SJ."],"DOI_URL":null},"PUB00080191":{"PMID":15520811,"ISBN":null,"volume":"5","issue":"11","year":2004,"title":"The LIM domain: from the cytoskeleton to the nucleus.","URL":null,"raw_pages":"920-31","medline_journal":"Nat Rev Mol Cell Biol","ISO_journal":"Nat. Rev. Mol. Cell Biol.","authors":["Kadrmas JL","Beckerle MC."],"DOI_URL":"http://dx.doi.org/10.1038/nrm1499"},"PUB00080209":{"PMID":7788499,"ISBN":null,"volume":"318","issue":"3","year":1995,"title":"LIM domain proteins.","URL":null,"raw_pages":"295-306","medline_journal":"C R Acad Sci III","ISO_journal":"C. R. Acad. Sci. III, Sci. Vie","authors":["Dawid IB","Toyama R","Taira M."],"DOI_URL":null},"PUB00071839":{"PMID":14647207,"ISBN":null,"volume":"4","issue":"12","year":2003,"title":"LIM-domain-binding protein 1: a multifunctional cofactor that interacts with diverse proteins.","URL":null,"raw_pages":"1132-7","medline_journal":"EMBO Rep","ISO_journal":"EMBO Rep.","authors":["Matthews JM","Visvader JE."],"DOI_URL":"http://dx.doi.org/10.1038/sj.embor.7400030"},"PUB00093154":{"PMID":20082688,"ISBN":null,"volume":"8","issue":null,"year":2010,"title":"Early evolution of the LIM homeobox gene family.","URL":null,"raw_pages":"4","medline_journal":"BMC Biol","ISO_journal":"BMC Biol.","authors":["Srivastava M","Larroux C","Lu DR","Mohanty K","Chapman J","Degnan BM","Rokhsar DS."],"DOI_URL":null},"PUB00007268":{"PMID":9594664,"ISBN":null,"volume":"14","issue":"4","year":1998,"title":"LIM domains: multiple roles as adapters and functional modifiers in protein interactions.","URL":null,"raw_pages":"156-62","medline_journal":"Trends Genet","ISO_journal":"Trends Genet.","authors":["Dawid IB","Breen JJ","Toyama R."],"DOI_URL":"http://dx.doi.org/10.1016/S0168-9525(98)01424-3"},"PUB00124088":{"PMID":16613990,"ISBN":null,"volume":"20","issue":"9","year":2006,"title":"The LIM-homeodomain proteins Isl-1 and Lhx3 act with steroidogenic factor 1 to enhance gonadotrope-specific activity of the gonadotropin-releasing hormone receptor gene promoter.","URL":null,"raw_pages":"2093-108","medline_journal":"Mol Endocrinol","ISO_journal":"Mol Endocrinol","authors":["Granger A","Bleux C","Kottler ML","Rhodes SJ","Counis R","Laverriere JN."],"DOI_URL":null},"PUB00124058":{"PMID":16022279,"ISBN":null,"volume":"32","issue":"2","year":2005,"title":"LIM-homeodomain genes in mammalian development and human disease.","URL":null,"raw_pages":"67-77","medline_journal":"Mol Biol Rep","ISO_journal":"Mol Biol Rep","authors":["Hunter CS","Rhodes SJ."],"DOI_URL":null},"PUB00124087":{"PMID":19337183,"ISBN":null,"volume":"6 Suppl 2","issue":null,"year":2009,"title":"LHX3 and LHX4 transcription factors in pituitary development and disease.","URL":null,"raw_pages":"283-90","medline_journal":"Pediatr Endocrinol Rev","ISO_journal":"Pediatr Endocrinol Rev","authors":["Colvin SC","Mullen RD","Pfaeffle RW","Rhodes SJ."],"DOI_URL":null},"PUB00125078":{"PMID":9865699,"ISBN":null,"volume":"95","issue":"6","year":1998,"title":"LIM homeodomain factors Lhx3 and Lhx4 assign subtype identities for motor neurons.","URL":null,"raw_pages":"817-28","medline_journal":"Cell","ISO_journal":"Cell","authors":["Sharma K","Sheng HZ","Lettieri K","Li H","Karavanov A","Potter S","Westphal H","Pfaff SL."],"DOI_URL":null},"PUB00125081":{"PMID":10598593,"ISBN":null,"volume":"13","issue":"12","year":1999,"title":"Differential activation of pituitary hormone genes by human Lhx3 isoforms with distinct DNA binding properties.","URL":null,"raw_pages":"2212-25","medline_journal":"Mol Endocrinol","ISO_journal":"Mol Endocrinol","authors":["Sloop KW","Meier BC","Bridwell JL","Parker GE","Schiller AM","Rhodes SJ."],"DOI_URL":null},"PUB00007267":{"PMID":10652534,"ISBN":null,"volume":"16","issue":"2","year":2000,"title":"Functions of LIM-homeobox genes.","URL":null,"raw_pages":"75-83","medline_journal":"Trends Genet","ISO_journal":"Trends Genet.","authors":["Hobert O","Westphal H."],"DOI_URL":"http://dx.doi.org/10.1016/S0168-9525(99)01883-1"},"PUB00080211":{"PMID":7954790,"ISBN":null,"volume":"79","issue":"2","year":1994,"title":"The LIM domain is a modular protein-binding interface.","URL":null,"raw_pages":"211-9","medline_journal":"Cell","ISO_journal":"Cell","authors":["Schmeichel KL","Beckerle MC."],"DOI_URL":"http://dx.doi.org/10.1016/0092-8674(94)90191-0"},"PUB00124086":{"PMID":12150931,"ISBN":null,"volume":"110","issue":"2","year":2002,"title":"LIM factor Lhx3 contributes to the specification of motor neuron and interneuron identity through cell-type-specific protein-protein interactions.","URL":null,"raw_pages":"237-49","medline_journal":"Cell","ISO_journal":"Cell","authors":["Thaler JP","Lee SK","Jurata LW","Gill GN","Pfaff SL."],"DOI_URL":null},"PUB00080210":{"PMID":17696879,"ISBN":null,"volume":"99","issue":"9","year":2007,"title":"The diverse biofunctions of LIM domain proteins: determined by subcellular localization and protein-protein interaction.","URL":null,"raw_pages":"489-502","medline_journal":"Biol Cell","ISO_journal":"Biol. Cell","authors":["Zheng Q","Zhao Y."],"DOI_URL":"http://dx.doi.org/10.1042/BC20060126"},"PUB00080208":{"PMID":12650064,"ISBN":null,"volume":"33","issue":"4","year":2002,"title":"[A review of the research progress on LIM protein family].","URL":null,"raw_pages":"305-8","medline_journal":"Sheng Li Ke Xue Jin Zhan","ISO_journal":"Sheng Li Ke Xue Jin Zhan","authors":["Zheng B","Wen JK","Han M."],"DOI_URL":null},"PUB00125082":{"PMID":11470784,"ISBN":null,"volume":"276","issue":"39","year":2001,"title":"An isoform-specific inhibitory domain regulates the LHX3 LIM homeodomain factor holoprotein and the production of a functional alternate translation form.","URL":null,"raw_pages":"36311-9","medline_journal":"J Biol Chem","ISO_journal":"J Biol Chem","authors":["Sloop KW","Dwyer CJ","Rhodes SJ."],"DOI_URL":null}},"set_info":{"accession":"cl02475","name":"LIM"},"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":0,"interactions":0,"matches":877,"pathways":0,"proteins":877,"proteomes":546,"sets":1,"structural_models":{"alphafold":787,"bfvd":0},"structures":1,"taxa":1772},"entry_annotations":{},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}