EC 22.214.171.124 - Lysine 2,3-aminomutase
IntEnz Enzyme Nomenclature
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. It contains pyridoxal phosphate and a [4Fe-4S] cluster and binds an exchangeable S-adenosyl-L-methionine molecule. Activity in vitro requires a strong reductant such as dithionite and strictly anaerobic conditions. A 5'-deoxyadenosyl radical is generated during the reaction cycle by reductive cleavage of S-adenosyl-L-methionine, mediated by the iron-sulfur cluster. S-adenosyl-L-methionine is regenerated at the end of the reaction.
Links to other databases
Stereochemistry of lysine 2,3-aminomutase.J. Am. Chem. Soc. 103: 6750-6752 (1981).
Studies on lysine-2,3-aminomutase. Subunit structure and sulfhydryl groups.Biochim. Biophys. Acta 207: 505-513 (1970). [PMID: 5452674]
Lysine 2,3-aminomutase: is adenosylmethionine a poor man's adenosylcobalamin?FASEB J. 7: 662-670 (1993). [PMID: 8500691]
S-Adenosylmethionine-dependent reduction of lysine 2,3-aminomutase and observation of the catalytically functional iron-sulfur centers by electron paramagnetic resonance.Biochemistry 37: 2578-2585 (1998). [PMID: 9485408]
The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale.Proc. Natl. Acad. Sci. U.S.A. 102: 13819-13824 (2005). [PMID: 16166264]
Pyridoxal-5'-phosphate as the catalyst for radical isomerization in reactions of PLP-dependent aminomutases.Biochim. Biophys. Acta 1814: 1548-1557 (2011). [PMID: 21435400]
[EC 126.96.36.199 created 1972]