EC - Glycoprotein endo-α-1,2-mannosidase

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IntEnz Enzyme Nomenclature


Accepted name:
glycoprotein endo-α-1,2-mannosidase
Other names:
glucosyl mannosidase
MANEA (gene name)
glycoprotein glucosylmannohydrolase
Systematic name:
glycoprotein glucosylmannohydrolase (configuration-retaining)



The enzyme catalyses the hydrolysis of the terminal α-D-glucosyl-(1→3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of N-glucosylated proteins during their processing in the Golgi apparatus. The name for the isomer is based on a nomenclature proposed by Prien et al [7].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ERGO , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004569


  1. Lubas, W.A. and Spiro, R.G.
    Golgi endo-α-D-mannosidase from rat liver, a novel N-linked carbohydrate unit processing enzyme.
    J. Biol. Chem. 262 : 3775-3781 (1987). [PMID: 3818665]
  2. Tulsiani, D.R.P., Coleman, V.P. and Touster, O.
    Asparagine-linked glycoprotein biosynthesis in rat brain: identification of glucosidase I, glucosidase II, and endomannosidase (glucosyl mannosidase).
    Arch. Biochem. Biophys. 277 : 114-121 (1990). [PMID: 2407194]
  3. Hiraizumi, S., Spohr, U., Spiro, R. G.
    Ligand affinity chromatographic purification of rat liver Golgi endomannosidase.
    J. Biol. Chem. 269 : 4697-4700 (1994). [PMID: 8106437]
  4. Spiro, M. J., Bhoyroo, V. D., Spiro, R. G.
    Molecular cloning and expression of rat liver endo-alpha-mannosidase, an N-linked oligosaccharide processing enzyme.
    J. Biol. Chem. 272 : 29356-29363 (1997). [PMID: 9361017]
  5. Hamilton, S. R., Li, H., Wischnewski, H., Prasad, A., Kerley-Hamilton, J. S., Mitchell, T., Walling, A. J., Davidson, R. C., Wildt, S., Gerngross, T. U.
    Intact {alpha}-1,2-endomannosidase is a typical type II membrane protein.
    Glycobiology 15 : 615-624 (2005). [PMID: 15677381]
  6. Hardt, B., Volker, C., Mundt, S., Salska-Navarro, M., Hauptmann, M., Bause, E.
    Human endo-alpha1,2-mannosidase is a Golgi-resident type II membrane protein.
    Biochimie 87 : 169-179 (2005). [PMID: 15760709]
  7. Prien, J. M., Ashline, D. J., Lapadula, A. J., Zhang, H., Reinhold, V. N.
    The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
    J. Am. Soc. Mass Spectrom. 20 : 539-556 (2009). [PMID: 19181540]

[EC created 1990, modified 2017]