EC - TRNA nucleotidyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
tRNA nucleotidyltransferase
Other names:
RNase PH
phosphate-dependent exonuclease
ribonuclease PH
Systematic name:
tRNA:phosphate nucleotidyltransferase



Brings about the final exonucleolytic trimming of the 3'-terminus of tRNA precursors in Escherichia coli by a phosphorolysis, producing a mature 3'-terminus on tRNA and nucleoside diphosphate. Not identical with EC polyribonucleotide nucleotidyltransferase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00983
Structural data: CSA , EC2PDB
Gene Ontology: GO:0009022
CAS Registry Number: 116412-36-3
UniProtKB/Swiss-Prot: (420) [show] [UniProt]


  1. Cudny, H. and Deutscher, M.P.
    3' processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement.
    J. Biol. Chem. 263: 1518-1523 (1988). [PMID: 3275667]
  2. Deutscher, M.P., Marshall, G.T. and Cudny, H.
    RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase.
    Proc. Natl. Acad. Sci. USA 85: 4710-4714 (1988). [PMID: 2455297]

[EC created 1992]