EC - α-1,6-mannosyl-glycoprotein 6-β-N-acetylglucosaminyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
α-1,6-mannosyl-glycoprotein 6-β-N-acetylglucosaminyltransferase
Other names:
N-acetylglucosaminyltransferase V
α-1,3(6)-mannosylglycoprotein β-1,6-N-acetylglucosaminyltransferase
α-mannoside β-1,6-N-acetylglucosaminyltransferase
UDP-N-acetylglucosamine:α-mannoside-β1,6 N-acetylglucosaminyltransferase
uridine diphosphoacetylglucosamine-α-mannoside β1→6-acetylglucosaminyltransferase
GlcNAc-T V
MGAT5 (gene name)
UDP-N-acetyl-D-glucosamine:6-[2-(N-acetyl-β-D-glucosaminyl)-α-D-mannosyl]-glycoprotein 6-β-N-acetyl-D-glucosaminyltransferase
Systematic name:
UDP-N-acetyl-α-D-glucosamine:N-acetyl-β-D-glucosaminyl-(1→2)-α-D-mannosyl-(1→6)-β-D-mannosyl-glycoprotein 6-β-N-acetyl-D-glucosaminyltransferase (configuration-inverting)



Requires Mg2+. The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. It catalyses the addition of N-acetylglucosamine in β 1-6 linkage to the α-linked mannose of biantennary N-linked oligosaccharides, and thus enables the synthesis of tri- and tetra-antennary complexes.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0030144
CAS Registry Number: 83588-90-3
UniProtKB/Swiss-Prot: (10) [show] [UniProt]


  1. Cummings, R.D., Trowbridge, I.S. and Kornfeld, S.
    A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: α-D-mannoside β1,6 N-acetylglucosaminyltransferase.
    J. Biol. Chem. 257 : 13421-13427 (1982). [PMID: 6216250]
  2. Hindsgaul, O., Tahir, S.H., Srivastava, O.P. and Pierce, M.
    The trisaccharide β-D-GlcpNAc-(1-2)-α-D-Manp-(1-6)-β-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V.
    Carbohydr. Res. 173 : 263-272 (1988). [PMID: 2834054]
  3. Shoreibah, M. G., Hindsgaul, O., Pierce, M.
    Purification and characterization of rat kidney UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6-N-acetylglucosaminyltransferase.
    J. Biol. Chem. 267 : 2920-2927 (1992). [PMID: 1531335]
  4. Gu, J., Nishikawa, A., Tsuruoka, N., Ohno, M., Yamaguchi, N., Kangawa, K., Taniguchi, N.
    Purification and characterization of UDP-N-acetylglucosamine: alpha-6-D-mannoside beta 1-6N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase V) from a human lung cancer cell line.
    J. Biochem. 113 : 614-619 (1993). [PMID: 8393437]
  5. Park, C., Jin, U. H., Lee, Y. C., Cho, T. J., Kim, C. H.
    Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B.
    Arch. Biochem. Biophys. 367 : 281-288 (1999). [PMID: 10395745]
  6. Saito, T., Miyoshi, E., Sasai, K., Nakano, N., Eguchi, H., Honke, K., Taniguchi, N.
    A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V distinct from the original glycosyltransferase activity.
    J. Biol. Chem. 277 : 17002-17008 (2002). [PMID: 11872751]

[EC created 1986, modified 2001, modified 2018]