EC 1.14.14.105 - Taxane 10β-hydroxylase

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IntEnz Enzyme Nomenclature
EC 1.14.14.105

Names

Accepted name:
taxane 10β-hydroxylase
Other names:
5-α-taxadienol-10-β-hydroxylase
CYP725A1
Systematic name:
taxa-4(20),11-dien-5α-yl acetate,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (10β-hydroxylating)

Reaction

Cofactor

Comments:

This microsomal cytochrome-P450 (heme-thiolate) enzyme from the plant Taxus cuspidata is involved in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel). Formerly EC 1.14.13.76.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00081
Structural data: CSA , EC2PDB
CAS Registry Number: 337514-75-7
UniProtKB/Swiss-Prot:

References

  1. Wheeler, A.L., Long, R.M., Ketchum, R.E., Rithner, C.D., Williams, R.M. and Croteau, R.
    Taxol biosynthesis: differential transformations of taxadien-5-α-ol and its acetate ester by cytochrome P450 hydroxylases from Taxus suspension cells.
    Arch. Biochem. Biophys. 390: 265-278 (2001). [PMID: 11396929]
  2. Schoendorf, A., Rithner, C. D., Williams, R. M., Croteau, R. B.
    Molecular cloning of a cytochrome P450 taxane 10 beta-hydroxylase cDNA from Taxus and functional expression in yeast.
    Proc. Natl. Acad. Sci. USA 98: 1501-1506 (2001). [PMID: 11171980]
  3. Schoendorf, A., Rithner, C. D., Williams, R. M., Croteau, R. B.
    Molecular cloning of a cytochrome P450 taxane 10 beta-hydroxylase cDNA from Taxus and functional expression in yeast.
    Proc. Natl. Acad. Sci. U.S.A. 98: 1501-1506 (2001). [PMID: 11171980]

[EC 1.14.14.105 created 2002 as EC 1.14.13.76, transferred 2018 to EC 1.14.14.105]