EC 1.14.14.89 - 4'-methoxyisoflavone 2'-hydroxylase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature
EC 1.14.14.89

Names

Accepted name:
4'-methoxyisoflavone 2'-hydroxylase
Other names:
isoflavone 2'-monooxygenase [ambiguous]
isoflavone 2'-hydroxylase [ambiguous]
CYP81E1
CYP81E3
CYP81E7
Systematic name:
formononetin,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)

Reaction

Cofactor

Comments:

A cytochrome P450 (heme-thiolate) protein. Acts on isoflavones with a 4'-methoxy group, such as formononetin and biochanin A. Involved in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain. EC 1.14.14.90, isoflavone 2'-hydroxylase, is less specific and acts on other isoflavones as well as 4'-methoxyisoflavones.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00081
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047957
CAS Registry Number: 110183-49-8
UniProtKB/Swiss-Prot:

References

  1. Hinderer, W., Flentje, U. and Barz, W.
    Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation.
    FEBS Lett. 214: 101-106 (1987).
  2. Akashi, T., Aoki, T., Ayabe, S.
    CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.
    Biochem. Biophys. Res. Commun. 251: 67-70 (1998). [PMID: 9790908]
  3. Liu, C. J., Huhman, D., Sumner, L. W., Dixon, R. A.
    Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula.
    Plant J. 36: 471-484 (2003). [PMID: 14617078]

[EC 1.14.14.89 created 1992 as EC 1.14.13.53, modified 2005, transferred 2018 to EC 1.14.14.89]