EC - Mannosyl-oligosaccharide α-1,3-glucosidase

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IntEnz Enzyme Nomenclature


Accepted name:
mannosyl-oligosaccharide α-1,3-glucosidase
Other names:
ER glucosidase II
α-glucosidase II
trimming glucosidase II
ROT2 (gene name)
GTB1 (gene name)
GANAB (gene name)
PRKCSH (gene name)
Systematic name:
Glc2Man9GlcNAc2-[protein] 3-α-glucohydrolase (configuration-inverting)



This eukaryotic enzyme cleaves off sequentially the two α-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature N-glycosylated proteins.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Trombetta, E. S., Simons, J. F., Helenius, A.
    Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit.
    J. Biol. Chem. 271: 27509-27516 (1996). [PMID: 8910335]
  2. Ziak, M., Meier, M., Etter, K. S., Roth, J.
    Two isoforms of trimming glucosidase II exist in mammalian tissues and cell lines but not in yeast and insect cells.
    Biochem. Biophys. Res. Commun. 280: 363-367 (2001). [PMID: 11162524]
  3. Wilkinson, B. M., Purswani, J., Stirling, C. J.
    Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulum.
    J. Biol. Chem. 281: 6325-6333 (2006). [PMID: 16373354]
  4. Mora-Montes, H. M., Bates, S., Netea, M. G., Diaz-Jimenez, D. F., Lopez-Romero, E., Zinker, S., Ponce-Noyola, P., Kullberg, B. J., Brown, A. J., Odds, F. C., Flores-Carreon, A., Gow, N. A.
    Endoplasmic reticulum alpha-glycosidases of Candida albicans are required for N glycosylation, cell wall integrity, and normal host-fungus interaction.
    Eukaryotic Cell 6: 2184-2193 (2007). [PMID: 17933909]

[EC created 2018]