EC 2.1.1.342 - Anaerobilin synthase

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IntEnz Enzyme Nomenclature
EC 2.1.1.342

Names

Accepted name:
anaerobilin synthase
Other name:
chuW (gene name)
Systematic name:
S-adenosyl-L-methionine:protoheme C-methyltransferase (anaerobilin-producing)

Reaction

Comments:

The enzyme, studied from the bacterium Escherichia coli O157:H7, is a radical SAM (AdoMet) enzyme that is involved in heme degradation and iron utilization under anaerobic conditions. The enzyme uses two SAM molecules for the reaction. The first molecule is used to generate a 5'-deoxyadenosyl radical, which abstracts a hydrogen atom from the methyl group of the second SAM molecule. The newly formed methylene radical attacks the substrate, causing a rearrangement of the porphyrin ring that results in the liberation of iron.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB

References

  1. LaMattina, J. W., Nix, D. B., Lanzilotta, W. N.
    Radical new paradigm for heme degradation in Escherichia coli O157:H7.
    Proc. Natl. Acad. Sci. U.S.A. 113: 12138-12143 (2016). [PMID: 27791000]
  2. LaMattina, J. W., Delrossi, M., Uy, K. G., Keul, N. D., Nix, D. B., Neelam, A. R., Lanzilotta, W. N.
    Anaerobic Heme Degradation: ChuY Is an Anaerobilin Reductase That Exhibits Kinetic Cooperativity.
    Biochemistry 56: 845-855 (2017). [PMID: 28045510]

[EC 2.1.1.342 created 2017]