EC 3.5.1.124 - Protein deglycase

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IntEnz Enzyme Nomenclature
EC 3.5.1.124

Names

Accepted name:
protein deglycase
Other names:
PARK7 (gene name)
DJ-1 protein
yhbO (gene name)
yajL (gene name)
glyoxylase III [incorrect]
Systematic name:
a [protein]-L-amino acid-1-hydroxypropan-2-one hydrolase [(R)-lactate-forming]

Reactions

Comments:

The enzyme, previously thought to be a glyoxalase, acts on glycated L-arginine, L-lysine, and L-cysteine residues within proteins that have been attacked and modified by glyoxal or 2-oxopropanal. The attack forms hemithioacetal in the case of cysteines and aminocarbinols in the case of arginines and lysines. The enzyme repairs the amino acids, releasing glycolate or lactate (70-80% (S)-lactate and 20-30% (R)-lactate), depending on whether the attacking agent was glyoxal or 2-oxopropanal, respectively.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (56) [show] [UniProt]

References

  1. Misra, K., Banerjee, A. B., Ray, S., Ray, M.
    Glyoxalase III from Escherichia coli: a single novel enzyme for the conversion of methylglyoxal into D-lactate without reduced glutathione.
    Biochem. J. 305: 999-1003 (1995). [PMID: 7848303]
  2. Subedi, K. P., Choi, D., Kim, I., Min, B., Park, C.
    Hsp31 of Escherichia coli K-12 is glyoxalase III.
    Mol. Microbiol. 81: 926-936 (2011). [PMID: 21696459]
  3. Richarme, G., Mihoub, M., Dairou, J., Bui, L. C., Leger, T., Lamouri, A.
    Parkinsonism-associated protein DJ-1/Park7 is a major protein deglycase that repairs methylglyoxal- and glyoxal-glycated cysteine, arginine, and lysine residues.
    J. Biol. Chem. 290: 1885-1897 (2015). [PMID: 25416785]
  4. Mihoub, M., Abdallah, J., Gontero, B., Dairou, J., Richarme, G.
    The DJ-1 superfamily member Hsp31 repairs proteins from glycation by methylglyoxal and glyoxal.
    Biochem. Biophys. Res. Commun. 463: 1305-1310 (2015). [PMID: 26102038]
  5. Abdallah, J., Mihoub, M., Gautier, V., Richarme, G.
    The DJ-1 superfamily members YhbO and YajL from Escherichia coli repair proteins from glycation by methylglyoxal and glyoxal.
    Biochem. Biophys. Res. Commun. 470: 282-286 (2016). [PMID: 26774339]

[EC 3.5.1.124 created 2016]