EC 1.14.19.49 - Tetracycline 7-halogenase

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IntEnz Enzyme Nomenclature
EC 1.14.19.49

Names

Accepted name:
tetracycline 7-halogenase
Other name:
ctcP (gene name)
Systematic name:
tetracycline:FADH2 oxidoreductase (7-halogenating)

Reaction

Comments:

The enzyme, characterized from the bacterium Streptomyces aureofaciens, is a member of the flavin-dependent halogenase family. The enzyme forms a lysine chloramine intermediate on an internal lysine residue before transferring the chlorine to the substrate. It is stereo-selective for the 4S (natural) isomer of tetracycline. FADH2 is provided by a dedicated EC 1.5.1.36, flavin reductase (NADH).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Dairi, T., Nakano, T., Aisaka, K., Katsumata, R., Hasegawa, M.
    Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline.
    Biosci. Biotechnol. Biochem. 59: 1099-1106 (1995). [PMID: 7612997]
  2. Zhu, T., Cheng, X., Liu, Y., Deng, Z., You, D.
    Deciphering and engineering of the final step halogenase for improved chlortetracycline biosynthesis in industrial Streptomyces aureofaciens.
    Metab. Eng. 19: 69-78 (2013). [PMID: 23800859]

[EC 1.14.19.49 created 2016]