EC - TRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase

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IntEnz Enzyme Nomenclature


Accepted name:
tRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase
Other names:
methylthio-threonylcarbamoyl-adenosine transferase B
CDKAL1 (gene name)
Systematic name:
tRNA (N6-L-threonylcarbamoyladenosine37):sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-(methylsulfanyl)transferase




The enzyme, which is a member of the S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes superfamily, binds two [4Fe-4S] clusters as well as the transferred sulfur. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0061712
UniProtKB/Swiss-Prot: (20) [show] [UniProt]


  1. Arragain, S., Handelman, S. K., Forouhar, F., Wei, F. Y., Tomizawa, K., Hunt, J. F., Douki, T., Fontecave, M., Mulliez, E., Atta, M.
    Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
    J. Biol. Chem. 285: 28425-28433 (2010). [PMID: 20584901]

[EC created 2014, modified 2015]