EC 2.8.4.5 - TRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase

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IntEnz Enzyme Nomenclature
EC 2.8.4.5

Names

Accepted name:
tRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase
Other names:
MtaB
methylthio-threonylcarbamoyl-adenosine transferase B
CDKAL1 (gene name)
Systematic name:
tRNA (N6-L-threonylcarbamoyladenosine37):sulfur-(sulfur carrier),S-adenosyl-L-methionine C2-(methylsulfanyl)transferase

Reaction

Cofactor

Comments:

The enzyme, which is a member of the S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes superfamily, binds two [4Fe-4S] clusters as well as the transferred sulfur. The sulfur donor is believed to be one of the [4Fe-4S] clusters, which is sacrificed in the process, so that in vitro the reaction is a single turnover. The identity of the electron donor is not known.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0061712
UniProtKB/Swiss-Prot: (20) [show] [UniProt]

References

  1. Arragain, S., Handelman, S. K., Forouhar, F., Wei, F. Y., Tomizawa, K., Hunt, J. F., Douki, T., Fontecave, M., Mulliez, E., Atta, M.
    Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
    J. Biol. Chem. 285: 28425-28433 (2010). [PMID: 20584901]

[EC 2.8.4.5 created 2014, modified 2015]