EC - α,α-trehalose synthase

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IntEnz Enzyme Nomenclature


Accepted name:
α,α-trehalose synthase
Other names:
trehalose synthase
trehalose synthetase
UDP-glucose:glucose 1-glucosyltransferase
ADP-glucose:D-glucose 1-α-D-glucosyltransferase
Systematic name:
NDP-α-D-glucose:D-glucose 1-α-D-glucosyltransferase




Requires Mg2+ for maximal activity [1]. The enzyme-catalysed reaction is reversible [1]. In the reverse direction to that shown above, the enzyme is specific for α,α-trehalose as substrate, as it cannot use α- or β-paranitrophenyl glucosides, maltose, sucrose, lactose or cellobiose [1]. While the enzymes from the thermophilic bacterium Rubrobacter xylanophilus and the hyperthermophilic archaeon Pyrococcus horikoshii can use ADP-, UDP- and GDP-α-D-glucose to the same extent [2,3], that from the hyperthermophilic archaeon Thermococcus litoralis has a marked preference for ADP-α-D-glucose [1] and that from the hyperthermophilic archaeon Thermoproteus tenax has a marked preference for UDP-α-D-glucose [4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102986


  1. Qu, Q., Lee, S.-J. and Boos, W.
    TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis.
    J. Biol. Chem. 279: 47890-47897 (2004). [PMID: 15364950]
  2. Ryu, S.-I., Park, C.-S., Cha, J., Woo, E.-J. and Lee, S.-B.
    A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: molecular cloning and characterization.
    Biochem. Biophys. Res. Commun. 329: 429-436 (2005). [PMID: 15737605]
  3. Nobre, A., Alarico, S., Fernandes, C., Empadinhas, N., da Costa, M. S.
    A unique combination of genetic systems for the synthesis of trehalose in Rubrobacter xylanophilus: properties of a rare actinobacterial TreT.
    J. Bacteriol. 190: 7939-7946 (2008). [PMID: 18835983]
  4. Kouril, T., Zaparty, M., Marrero, J., Brinkmann, H., Siebers, B.
    A novel trehalose synthesizing pathway in the hyperthermophilic Crenarchaeon Thermoproteus tenax: the unidirectional TreT pathway.
    Arch. Microbiol. 190: 355-369 (2008). [PMID: 18483808]

[EC created 2008, modified 2013]