EC - Malonyl-[acyl-carrier protein] O-methyltransferase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
malonyl-[acyl-carrier protein] O-methyltransferase
Other name:
Systematic name:
S-adenosyl-L-methionine:malonyl-[acyl-carrier protein] O-methyltransferase



Involved in an early step of biotin biosynthesis in Gram-negative bacteria. This enzyme catalyses the transfer of a methyl group to the ω-carboxyl group of malonyl-[acyl-carrier protein] forming a methyl ester. The methyl ester is recognized by the fatty acid synthetic enzymes, which process it via the fatty acid elongation cycle to give pimelyl-[acyl-carrier-protein] methyl ester [5]. While the enzyme can also accept malonyl-CoA, it has a much higher activity with malonyl-[acyl-carrier protein] [6].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102130
UniProtKB/Swiss-Prot: (58) [show] [UniProt]


  1. Del Campillo-Campbell, A., Kayajanian, G., Campbell, A., Adhya, S.
    Biotin-requiring mutants of Escherichia coli K-12.
    J. Bacteriol. 94: 2065-2066 (1967). [PMID: 4864413]
  2. Rolfe, B., Eisenberg, M. A.
    Genetic and biochemical analysis of the biotin loci of Escherichia coli K-12.
    J. Bacteriol. 96: 515-524 (1968). [PMID: 4877129]
  3. Otsuka, A. J., Buoncristiani, M. R., Howard, P. K., Flamm, J., Johnson, C., Yamamoto, R., Uchida, K., Cook, C., Ruppert, J., Matsuzaki, J.
    The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon.
    J. Biol. Chem. 263: 19577-19585 (1988). [PMID: 3058702]
  4. Cleary, P. P., Campbell, A.
    Deletion and complementation analysis of biotin gene cluster of Escherichia coli.
    J. Bacteriol. 112: 830-839 (1972). [PMID: 4563978]
  5. Lin, S., Hanson, R. E., Cronan, J. E.
    Biotin synthesis begins by hijacking the fatty acid synthetic pathway.
    Nat. Chem. Biol. 6: 682-688 (2010). [PMID: 20693992]
  6. Lin, S., Cronan, J. E.
    The BioC O-methyltransferase catalyzes methyl esterification of malonyl-acyl carrier protein, an essential step in biotin synthesis.
    J. Biol. Chem. 287: 37010-37020 (2012). [PMID: 22965231]

[EC created 2010, modified 2013]