EC 6.2.1.39 - [butirosin acyl-carrier protein]—L-glutamate ligase
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ENZYME view
IntEnz Enzyme Nomenclature
EC 6.2.1.39
Names
Accepted name:
[butirosin acyl-carrier protein]—L-glutamate ligase
Other
names:
[BtrI acyl-carrier protein]—L-glutamate ligase
BtrJ
BtrJ
Systematic name:
[BtrI acyl-carrier protein]:L-glutamate ligase (ADP-forming)
Reactions
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53948 [IUBMB]ATPName origin: UniProt - CHECKED (C)Formula: C10H12N5O13P3
Charge: -4ChEBI compound status: CHECKED (C)holo-[BtrI ACP]GENERIC:13741Is ROOT: no
ROOT compound: GENERIC:13739Number of residues: 1L-glutamateName origin: UniProt - CHECKED (C)Formula: C5H8NO4
Charge: -1ChEBI compound status: CHECKED (C)=ADPName origin: UniProt - CHECKED (C)Formula: C10H12N5O10P2
Charge: -3ChEBI compound status: CHECKED (C)γ-L-glutamyl-[BtrI ACP]GENERIC:13742Is ROOT: no
ROOT compound: GENERIC:13739Number of residues: 1 -
53952 [IUBMB]4-aminobutanoyl-[BtrI ACP]GENERIC:13744Is ROOT: no
ROOT compound: GENERIC:13739Number of residues: 1ATPName origin: UniProt - CHECKED (C)Formula: C10H12N5O13P3
Charge: -4ChEBI compound status: CHECKED (C)L-glutamateName origin: UniProt - CHECKED (C)Formula: C5H8NO4
Charge: -1ChEBI compound status: CHECKED (C)=4-(γ-L-glutamylamino)butanoy-[BtrI ACP]GENERIC:13745Is ROOT: no
ROOT compound: GENERIC:13739Number of residues: 1ADPName origin: UniProt - CHECKED (C)Formula: C10H12N5O10P2
Charge: -3ChEBI compound status: CHECKED (C)H+Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C)
Cofactor
Comments:
Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule. Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
Links to other databases
UniProtKB/Swiss-Prot:
BTRJ_BACCI
References
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Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.Chem. Biol. 12 : 665-675 (2005). [PMID: 15975512]
[EC 6.2.1.39 created 2012]