IntEnz

EC 6.2.1.39 - [butirosin acyl-carrier protein]—L-glutamate ligase

IntEnz Enzyme Nomenclature
EC 6.2.1.39

Names

Reactions

• 53948 [IUBMB]
  ATP

  ATP

  Name origin: UniProt - CHECKED (C)

  CHEBI:30616

  Formula: C10H12N5O13P3
  Charge: -4

  ChEBI compound status: CHECKED (C)

  

  +

  holo-[BtrI ACP]

  holo-[BtrI ACP]

  GENERIC:13741

  Is ROOT: no
  ROOT compound: GENERIC:13739

  Number of residues: 1

  O-(pantetheine-4ʼ-phosphoryl)-L-serine residue O-(pantetheine-4'-phosphoryl)-L-serine residue Name origin: UniProt - CHECKED (C) CHEBI:64479 Formula: C14H25N3O8PS Charge: -1 Position: undefined ChEBI compound status: CHECKED (C)

  +

  L-glutamate

  L-glutamate

  Name origin: UniProt - CHECKED (C)

  CHEBI:29985

  Formula: C5H8NO4
  Charge: -1

  ChEBI compound status: CHECKED (C)

  

  =

  ADP

  ADP

  Name origin: UniProt - CHECKED (C)

  CHEBI:456216

  Formula: C10H12N5O10P2
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

  +

  L-glutamyl-[BtrI ACP]

  L-glutamyl-[BtrI ACP]

  GENERIC:13742

  Is ROOT: no
  ROOT compound: GENERIC:13739

  Number of residues: 1

  O-[S-γ-L-glutamylpantetheine-4'-phosphoryl]serine residue O-[S-gamma-L-glutamylpantetheine-4'-phosphoryl]serine residue Name origin: UniProt - CHECKED (C) CHEBI:137996 Formula: C19H32N4O11PS Charge: -1 Position: undefined ChEBI compound status: CHECKED (C)

  +

  phosphate

  phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:43474

  Formula: HO4P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  
• 53952 [IUBMB]
  4-aminobutanoyl-[BtrI ACP]

  4-aminobutanoyl-[BtrI ACP]

  GENERIC:13744

  Is ROOT: no
  ROOT compound: GENERIC:13739

  Number of residues: 1

  O-[S-4-aminobutanoylpantetheine-4'-phosphoryl]serine residue O-[S-4-aminobutanoylpantetheine-4'-phosphoryl]serine residue Name origin: UniProt - CHECKED (C) CHEBI:137997 Formula: C18H33N4O9PS Charge: 0 Position: undefined ChEBI compound status: CHECKED (C)

  +

  ATP

  ATP

  Name origin: UniProt - CHECKED (C)

  CHEBI:30616

  Formula: C10H12N5O13P3
  Charge: -4

  ChEBI compound status: CHECKED (C)

  

  +

  L-glutamate

  L-glutamate

  Name origin: UniProt - CHECKED (C)

  CHEBI:29985

  Formula: C5H8NO4
  Charge: -1

  ChEBI compound status: CHECKED (C)

  

  =

  4-(γ-L-glutamylamino)butanoy-[BtrI ACP]

  4-(gamma-L-glutamylamino)butanoy-[BtrI ACP]

  GENERIC:13745

  Is ROOT: no
  ROOT compound: GENERIC:13739

  Number of residues: 1

  O-[S-4-(γ-L-glutamylamino)butanoylpantetheine-4'-phosphoryl]serine residue O-[S-4-(gamma-L-glutamylamino)butanoylpantetheine-4'-phosphoryl]serine residue Name origin: UniProt - CHECKED (C) CHEBI:137998 Formula: C23H39N5O12PS Charge: -1 Position: undefined ChEBI compound status: CHECKED (C)

  +

  ADP

  ADP

  Name origin: UniProt - CHECKED (C)

  CHEBI:456216

  Formula: C10H12N5O10P2
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  phosphate

  phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:43474

  Formula: HO4P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

Cofactor

• Mn²⁺ or Mg²⁺

Comments:

Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule. Requires Mg²⁺ or Mn²⁺, and activity is enhanced in the presence of Mn²⁺.

Links to other databases

References

1. Li, Y., Llewellyn, N. M., Giri, R., Huang, F., Spencer, J. B.
   Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.
   Chem. Biol. 12: 665-675 (2005). [PMID: 15975512]

[EC 6.2.1.39 created 2012]