EC - TRNA (guanine26-N2)-dimethyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
tRNA (guanine26-N2)-dimethyltransferase
Other names:
tRNA (m22G26)dimethyltransferase
Systematic name:
S-adenosyl-L-methionine:tRNA (guanine26-N2)-dimethyltransferase



The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions. In contrast to EC, tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase, this enzyme does not catalyse the methylation of guanine27 in tRNA.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (61) [show] [UniProt]


  1. Constantinesco, F., Motorin, Y., Grosjean, H.
    Characterisation and enzymatic properties of tRNA(guanine26, N2,N2)-dimethyltransferase (Trm1p) from Pyrococcus furiosus.
    J. Mol. Biol. 291: 375-392 (1999). [PMID: 10438627]
  2. Constantinesco, F., Benachenhou, N., Motorin, Y., Grosjean, H.
    The tRNA(guanine-26,N2,N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli.
    Nucleic Acids Res. 26: 3753-3761 (1998). [PMID: 9685492]
  3. Liu, J., Liu, J., Stråby, K. B.
    Point and deletion mutations eliminate one or both methyl group transfers catalysed by the yeast TRM1 encoded tRNA (m22G26)dimethyltransferase.
    Nucleic Acids Res. 26: 5102-5108 (1998). [PMID: 9801306]
  4. Liu, J., Zhou, G. Q., Stråby, K. B.
    Caenorhabditis elegans ZC376.5 encodes a tRNA (m22G26)dimethyltransferance in which 246arginine is important for the enzyme activity.
    Gene 226: 73-81 (1999). [PMID: 10048958]

[EC, created 1972 as EC, part-transferred 2011 to EC]