EC 126.96.36.199 - Fluoroacetyl-CoA thioesterase
IntEnz Enzyme Nomenclature
28542 [IUBMB]fluoroacetyl-CoAfluoroacetyl-CoAName origin: UniProt - CHECKED (C)Formula: C23H33FN7O17P3S
Charge: -4ChEBI compound status: CHECKED (C)H2OH2OName origin: UniProt - CHECKED (C)Formula: H2O
Charge: 0ChEBI compound status: CHECKED (C)=CoACoAName origin: UniProt - CHECKED (C)Formula: C21H32N7O16P3S
Charge: -4ChEBI compound status: CHECKED (C)fluoroacetatefluoroacetateName origin: UniProt - CHECKED (C)Formula: C2H2FO2
Charge: -1ChEBI compound status: CHECKED (C)
Fluoroacetate is extremely toxic. It reacts with CoA to form fluoroacetyl-CoA, which substitutes for acetyl CoA and reacts with EC 188.8.131.52 (citrate synthase) to produce fluorocitrate, a metabolite of which binds very tightly to EC 184.108.40.206 (aconitase) and halts the TCA cycle. This enzyme hydrolyses fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance on the organisms that produce it. It has been described in the poisonous plant Dichapetalum cymosum and the bacterium Streptomyces cattleya, both of which are fluoroacetate producers.
Links to other databases
Fluoroacetyl-coenzyme-A hydrolase-like activity in Dichapetalum cymosum.J. Plant Physiol. 139: 369-372 (1992).
The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A.Chem. Biol. 13: 475-484 (2006). [PMID: 16720268]
Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK.J. Biol. Chem. 285: 22495-22504 (2010). [PMID: 20430898]
[EC 220.127.116.11 created 2011]