EC - TRNA pseudouridine38-40 synthase

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IntEnz Enzyme Nomenclature


Accepted name:
tRNA pseudouridine38-40 synthase
Other names:
tRNA pseudouridylate synthase I
Systematic name:
tRNA-uridine38-40 uracil mutase



The uridylate residues at positions 38, 39 and 40 of nearly all tRNAs are isomerized to pseudouridine. TruA specifically modifies uridines at positions 38, 39, and/or 40 in the anticodon stem loop of tRNAs with highly divergent sequences and structures [1].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0106029
UniProtKB/Swiss-Prot: (630) [show] [UniProt]


  1. Hur, S., Stroud, R. M.
    How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA.
    Mol. Cell 26: 189-203 (2007). [PMID: 17466622]
  2. Huang, L., Pookanjanatavip, M., Gu, X., Santi, D. V.
    A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst.
    Biochemistry 37: 344-351 (1998). [PMID: 9425056]
  3. Kammen, H.O., Marvel, C.C., Hardy, L. and Penhoet, E.E.
    Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I.
    J. Biol. Chem. 263: 2255-2263 (1988). [PMID: 3276686]
  4. Turnbough, C. L., Neill, R. J., Landsberg, R., Ames, B. N.
    Pseudouridylation of tRNAs and its role in regulation in Salmonella typhimurium.
    J. Biol. Chem. 254: 5111-5119 (1979). [PMID: 376505]
  5. Zhao, X., Horne, D. A.
    The role of cysteine residues in the rearrangement of uridine to pseudouridine catalyzed by pseudouridine synthase I.
    J. Biol. Chem. 272: 1950-1955 (1997). [PMID: 8999885]
  6. Foster, P. G., Huang, L., Santi, D. V., Stroud, R. M.
    The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I.
    Nat. Struct. Biol. 7: 23-27 (2000). [PMID: 10625422]
  7. Dong, X., Bessho, Y., Shibata, R., Nishimoto, M., Shirouzu, M., Kuramitsu, S., Yokoyama, S.
    Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8.
    RNA Biol 3: 115-122 (2006). [PMID: 17114947]
  8. Arena, F., Ciliberto, G., Ciampi, S. and Cortese, R.
    Pseudouridylation of tRNAs and its role in regulation in Salmonella typhimurium.
    J. Biol. Chem. 5: 4523-4536 (1978). [PMID: 376505]

[EC created 1990, modified 2011]