EC 2.5.1.71 - Leachianone-G 2''-dimethylallyltransferase

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IntEnz Enzyme Nomenclature
EC 2.5.1.71

Names

Accepted name:
leachianone-G 2''-dimethylallyltransferase
Other names:
LG 2''-dimethylallyltransferase
leachianone G 2''-dimethylallyltransferase
LGDT
Systematic name:
dimethylallyl-diphosphate:leachianone-G 2''-dimethylallyltransferase

Reaction

Cofactor

Comments:

This membrane-bound enzyme is located in the plastids and requires Mg2+ for activity. The reaction forms the lavandulyl sidechain of sophoraflavanone G by transferring a dimethylallyl group to the 2'' position of another dimethylallyl group attached at postiion 8 of leachianone G. The enzyme is specific for dimethylallyl diphosphate as the prenyl donor, as it cannot be replaced by isopentenyl diphosphate or geranyl diphosphate. Euchrenone a7 (a 5-deoxy derivative of leachianone G) and kenusanone I (a 7-methoxy derivative of leachianone G) can also act as substrates, but more slowly. Along with EC 1.14.13.103 (8-dimethylallylnaringenin 2'-hydroxylase) and EC 2.5.1.70 (naringenin 8-dimethylallyltransferase), this enzyme forms part of the sophoraflavanone-G-biosynthesis pathway.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB

References

  1. Zhao, P., Inoue, K., Kouno, I. and Yamamoto, H.
    Characterization of leachianone G 2′′-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures.
    Plant Physiol. 133: 1306-1313 (2003). [PMID: 14551337]

[EC 2.5.1.71 created 2007]