EC - trans-aconitate 2-methyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
trans-aconitate 2-methyltransferase
Systematic name:
S-adenosyl-L-methionine:(E)-prop-1-ene-1,2,3-tricarboxylate 2'-O-methyltransferase



Also catalyses the formation of the methyl monoester of cis-aconitate, isocitrate and citrate, but more slowly. While the enzyme from Escherichia coli forms (E)-3-(methoxycarbonyl)-pent-2-enedioate as the product, that from Saccharomyces cerevisiae forms (E)-2-(methoxycarbonylmethyl)butenedioate and is therefore classified as a separate enzyme (cf. EC, trans-aconitate 3-methyltransferase).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0030798
UniProtKB/Swiss-Prot: (90) [show] [UniProt]


  1. Cai, H. and Clarke, S.
    A novel methyltransferase catalyzes the esterification of trans-aconitate in Escherichia coli.
    J. Biol. Chem. 274: 13470-13479 (1999). [PMID: 10224113]
  2. Cai, H., Strouse, J., Dumlao, D., Jung, M.E. and Clarke, S.
    Distinct reactions catalyzed by bacterial and yeast trans-aconitate methyltransferase.
    Biochemistry 40: 2210-2219 (2001). [PMID: 11329290]
  3. Cai, H., Dumlao, D., Katz, J.E. and Clarke, S.
    Identification of the gene and characterization of the activity of the trans-aconitate methyltransferase from Saccharomyces cerevisiae.
    Biochemistry 40: 13699-13709 (2001). [PMID: 11695919]

[EC created 2002]