EC 7.5.2.5 - ABC-type lipopolysaccharide transporter

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IntEnz Enzyme Nomenclature
EC 7.5.2.5

Names

Accepted name:
ABC-type lipopolysaccharide transporter
Other names:
lptB (gene name)
lipopolysaccharide transport system
lipopolysaccharide-transporting ATPase
Systematic name:
ATP phosphohydrolase (ABC-type, lipopolysaccharide-exporting)

Reaction

Comments:

An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. Does not undergo phosphorylation during the transport process. The enzyme, characterized from the bacterium Escherichia coli, functions as part of the lipopolysaccharide (LPS) export system, a seven protein system that translocates LPS from the inner- to the outer membrane. The ATPase activity in this system is implicated in releasing LPS from the inner membrane. Formerly EC 3.6.3.39.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00185
Structural data: CSA , EC2PDB
Gene Ontology: GO:0015437
UniProtKB/Swiss-Prot:

References

  1. Sperandeo, P., Cescutti, R., Villa, R., Di Benedetto, C., Candia, D., Deho, G., Polissi, A.
    Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli.
    J. Bacteriol. 189: 244-253 (2007). [PMID: 17056748]
  2. Ruiz, N., Gronenberg, L. S., Kahne, D., Silhavy, T. J.
    Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli.
    Proc. Natl. Acad. Sci. U.S.A. 105: 5537-5542 (2008). [PMID: 18375759]
  3. Narita, S., Tokuda, H.
    Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides.
    FEBS Lett. 583: 2160-2164 (2009). [PMID: 19500581]
  4. Tran, A. X., Dong, C., Whitfield, C.
    Structure and functional analysis of LptC, a conserved membrane protein involved in the lipopolysaccharide export pathway in Escherichia coli.
    J. Biol. Chem. 285: 33529-33539 (2010). [PMID: 20720015]
  5. Okuda, S., Freinkman, E., Kahne, D.
    Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in E. coli.
    Science 338: 1214-1217 (2012). [PMID: 23138981]
  6. Chng, S. S., Gronenberg, L. S., Kahne, D.
    Proteins required for lipopolysaccharide assembly in Escherichia coli form a transenvelope complex.
    Biochemistry 49: 4565-4567 (2010). [PMID: 20446753]

[EC 7.5.2.5 created 2000 as EC 3.6.3.39, transferred 2018 to EC 7.5.2.5]