EC - Biotin—[biotin carboxyl-carrier protein] ligase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
biotin—[biotin carboxyl-carrier protein] ligase
Other names:
HCS1 (gene name)
acetyl CoA holocarboxylase synthetase
acetyl coenzyme A holocarboxylase synthetase
biotin holoenzyme synthetase
biotin-[acetyl coenzyme A carboxylase] synthetase
biotin-[acetyl-CoA carboxylase] synthetase
biotin:apocarboxylase ligase
biotin—protein ligase
biotin—[acetyl-CoA carboxylase] synthetase
acetyl-CoA carboxylase biotin holoenzyme synthetase
birA (gene name)
HLCS (gene name)
biotin:apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ligase (AMP-forming)
biotin—[acetyl-CoA-carboxylase] ligase
Systematic name:
biotin:apo-[carboxyl-carrier protein] ligase (AMP-forming)



The enzyme biotinylates a biotin carboxyl-carrier protein that is part of an acetyl-CoA carboxylase complex, enabling its subsequent carboxylation by EC, biotin carboxylase. The carboxyl group is eventually transferred to acetyl-CoA by EC, acetyl-CoA carboxytransferase. In some organisms the carrier protein is part of EC, acetyl-CoA carboxylase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004077
CAS Registry Number: 37340-95-7
UniProtKB/Swiss-Prot: (18) [show] [UniProt]


  1. Landman, A.D. and Dakshinamurti, K.
    Acetyl-Coenzyme A carboxylase. Role of the prosthetic group in enzyme polymerization.
    Biochem. J. 145 : 545-548 (1975). [PMID: 239688]
  2. Wilson, K. P., Shewchuk, L. M., Brennan, R. G., Otsuka, A. J., Matthews, B. W.
    Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains.
    Proc. Natl. Acad. Sci. U.S.A. 89 : 9257-9261 (1992). [PMID: 1409631]
  3. Nenortas, E., Beckett, D.
    Purification and characterization of intact and truncated forms of the Escherichia coli biotin carboxyl carrier subunit of acetyl-CoA carboxylase.
    J. Biol. Chem. 271 : 7559-7567 (1996). [PMID: 8631788]

[EC created 1978, modified 2018]