EC 5.6.1.9 - (R)-2-hydroxyacyl-CoA dehydratase activating ATPase

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IntEnz Enzyme Nomenclature
EC 5.6.1.9

Names

Accepted name:
(R)-2-hydroxyacyl-CoA dehydratase activating ATPase
Other names:
(R)-2-hydroxyacyl-CoA dehydratase activase
archerase
Systematic name:
reduced flavodoxin:(R)-2-hydroxyacyl-CoA dehydratase electron transferase (ATP-hydrolyzing)

Reaction

Comments:

Members of the (R)-2-hydroxyacyl-CoA dehydratase family (including EC 4.2.1.54, lactoyl-CoA dehydratase, EC 4.2.1.157, (R)-2-hydroxyisocaproyl-CoA dehydratase, EC 4.2.1.167, (R)-2-hydroxyglutaryl-CoA dehydratase and EC 4.2.1.175, (R)-3-(aryl)lactoyl-CoA dehydratase) are two-component systems composed of an activator component and a dehydratase component. The activator is an extremely oxygen-sensitive homodimer with one [4Fe-4S] cluster bound at the dimer interface. Before it can catalyse the dehydration reaction, the dehydratase requires one high-energy electron that is used to transiently reduce the electrophilic thiol ester carbonyl to a nucleophilic ketyl radical anion, facilitating the elimination of the hydroxyl group. The activator, which has been named archerase because its open position resembles an archer shooting arrows, binds two ADP molecules. Upon the reduction of its [4Fe-4S] cluster by a single electron, delivered by a dedicated flavodoxin or a clostridial ferredoxin, the two ADP molecules exchange for two ATP molecules, resulting in a large conformational change. The change allows the activator to bind to the dehydratase component and transfer the electron to it, activating it. During this event the two ATP molecules are hydrolysed and the activator returns to its resting state. Since the electron is regenerated at the end of each reaction cycle of the dehydratase, the activation is required only once, before the first reaction takes place.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB

References

  1. Bendrat, K., Muller, U., Klees, A. G., Buckel, W.
    Identification of the gene encoding the activator of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans by gene expression in Escherichia coli.
    FEBS Lett. 329 : 329-331 (1993). [PMID: 8365476]
  2. Muller, U., Buckel, W.
    Activation of (R)-2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans.
    Eur. J. Biochem. 230 : 698-704 (1995). [PMID: 7607244]
  3. Locher, K. P., Hans, M., Yeh, A. P., Schmid, B., Buckel, W., Rees, D. C.
    Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A.
    J. Mol. Biol. 307 : 297-308 (2001). [PMID: 11243821]
  4. Dickert, S., Pierik, A. J., Buckel, W.
    Molecular characterization of phenyllactate dehydratase and its initiator from Clostridium sporogenes.
    Mol. Microbiol. 44 : 49-60 (2002). [PMID: 11967068]
  5. Thamer, W., Cirpus, I., Hans, M., Pierik, A. J., Selmer, T., Bill, E., Linder, D., Buckel, W.
    A two [4Fe-4S]-cluster-containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans.
    Arch. Microbiol. 179 : 197-204 (2003). [PMID: 12610725]
  6. Kim, J., Hetzel, M., Boiangiu, C. D., Buckel, W.
    Dehydration of (R)-2-hydroxyacyl-CoA to enoyl-CoA in the fermentation of alpha-amino acids by anaerobic bacteria.
    FEMS Microbiol. Rev. 28 : 455-468 (2004). [PMID: 15374661]
  7. Kim, J., Darley, D., Buckel, W.
    2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile.
    FEBS J. 272 : 550-561 (2005). [PMID: 15654892]
  8. Kim, J., Darley, D. J., Buckel, W., Pierik, A. J.
    An allylic ketyl radical intermediate in clostridial amino-acid fermentation.
    Nature 452 : 239-242 (2008). [PMID: 18337824]
  9. Knauer, S. H., Buckel, W., Dobbek, H.
    On the ATP-dependent activation of the radical enzyme (R)-2-hydroxyisocaproyl-CoA dehydratase.
    Biochemistry 51 : 6609-6622 (2012). [PMID: 22827463]

[EC 5.6.1.9 created 2019]