EC 5 - Isomerases
EC 5.3 - Intramolecular oxidoreductases
EC 5.3.1 - Interconverting aldoses and ketoses
EC 5.3.1.4 - L-arabinose isomerase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 5.3.1.4
Names
Accepted name:
L-arabinose isomerase
Other
name:
L-arabinose ketol-isomerase
Systematic name:
α-L-arabinose aldose-ketose-isomerase
Reaction
- beta-L-arabinose = L-ribulose
Cofactor
Comments:
Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+) [2]. The enzyme binds β-L-arabinopyranose [4] and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism [5]. The enzyme can also convert α-D-galactose to D-tagatose with lower efficiency [4].
Links to other databases
Gene Ontology:
GO:0008733
CAS Registry Number:
9023-80-7
References
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Pentose formation by Lactobacillus plantarum. II. L-Arabinose isomerase.J. Biol. Chem. 231 : 1031-1037 (1958). [PMID: 13539034]
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Purification and properties of an L-arabinose isomerase from Escherichia coli.J. Biol. Chem. 243 : 4312-4318 (1968). [PMID: 4878429]
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Crystallization and properties of L-arabinose isomerase from Lactobacillus gayonii.Biochim. Biophys. Acta 178 : 156-165 (1969). [PMID: 5773448]
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Bioconversion of D-galactose into D-tagatose.Enzyme Microb. Technol. 15 : 105-108 (1993).
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The evolution of sugar isomerases.Protein Eng. 8 : 1189-1195 (1995). [PMID: 8869631]
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Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative target of biological tagatose production.J. Mol. Biol. 360 : 297-309 (2006). [PMID: 16756997]
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Anomeric specificity and mechanism of two pentose isomerases.Biochemistry 10 : 1058-1062 (1971). [PMID: 5550812]
[EC 5.3.1.4 created 1961]